Gilles Gouspillou

Mechanisms of protein degradation in atrophying muscles: what have we learned during the past decade? Skeletal muscle atrophy occurs in diverse conditions, including aging, disuse, cancer cachexia, and chronic disease. It results from an imbalance between protein synthesis and degradation, where exces...

Mechanisms of protein degradation in atrophying muscles: what have we learned during the past decade?
www.jbc.org/article/S002...

Canonical and non‐canonical functions of proteins regulating mitochondrial dynamics in mammalian physiology Abstract figure legend Mitochondria are highly dynamic organelles that continuously remodel their architecture through coordinated cycles of fusion and fission. This review examines the four key GTPa...

Mitochondrial dynamics proteins have other functions than just regulating mito dynamics, but they are often forgotten.

Congrats to the team and especially Rémi Chaney and @gillesgouspillou.bsky.social who did the heavy lifting on this review 💪

physoc.onlinelibrary.wiley.com/doi/10.1113/...

A 12‐Week Strength Training Improves Mitochondrial Respiration, H2O2 Emission and Skeletal Muscle Integrity in Women With Myotonic Dystrophy Type 1 Background Myotonic dystrophy type 1 (DM1) is caused by expanded CTG repeats in the DMPK gene, causing the accumulation of toxic RNA that sequesters RNA-binding proteins. Clinically, DM1 is characte.....

Strength training improves mitochondrial bioenergetics and skeletal muscle integrity in women with Myotonic Dystrophy Type 1.

Thanks to everyone involved, notably @gillesgouspillou.bsky.social, @jpleduc-gaudet.bsky.social, E. Duchesne, L. Girard-Coté.

onlinelibrary.wiley.com/doi/10.1111/...

NSAID ingestion augments training‐induced muscle hypertrophy and differentially affects muscle mRNA expression, but not strength gains, in trained men Abstract figure legend Schematic outlining the impact of NSAID ingestion on resistance exercise training-induced changes in muscle morphology, function and gene networks relative to placebo ingestion...

Hopefully of interest to scientists and exercise practitioners with and interest in muscle mass and functional gains in human volunteers. doi.org/10.1113/JP28...

@jphysiol.bsky.social

Impact of physical activity on physical function, mitochondrial energetics, ROS production, and Ca2+ handling across the adult lifespan in men Cefis et al. show that mitochondrial respiration and ROS production are not affected during healthy muscle aging but identify altered mitochondrial calcium handling as a potential key driving mechanis...

Excited to share our latest paper, out in
@cp-cellrepmed.bsky.social, investigating the impact of physical activity on physical function, mitochondrial energetics, ROS production, and Ca2+ handling across the adult lifespan in men. Spearheaded by M Cefis & V Marcangeli
www.cell.com/cell-reports...

Experimental Evidence Against Taurine Deficiency as a Driver of Aging in Humans Circulating taurine concentration is not associated with age, muscle mass, strength, physical performance, or mitochondrial function in humans. Taurine deficiency is therefore unlikely to be a primar....

Experimental Evidence Against Taurine Deficiency as a Driver of Aging in Humans
onlinelibrary.wiley.com/doi/full/10....

Dysregulated skeletal muscle myosin super-relaxation and energetics in male participants with type 2 diabetes mellitus - Diabetologia Aims/hypothesis Disrupted energy balance is critical for the onset and development of type 2 diabetes mellitus. Understanding of the exact underlying metabolic mechanisms remains incomplete, but skele...

Our new very collaborative paper showing that muscle myosin has a reduced energy demand in diabetes. Big impact on weight control in our mind!!! Thank you to everyone involved and funders @lundbeckfonden.bsky.social @novo-nordisk.bsky.social @ucph.bsky.social @myoblue.bsky.social #diabetes

📣 CALL FOR PAPERS 📣

Organised by @pgreenhaff.bsky.social & focusing on:

🚑 Injury and rehabilitation
⛰️ Altitude physiology
🦵 Muscle adaptation
🚴 Exercise training
💉 Pharmacological interventions
⚙️ Body composition

🔗 buff.ly/2sVbnLk

Retromer promotes the lysosomal turnover of mtDNA Lysosomal uptake of mtDNA controls mitochondrial quality.

www.science.org/doi/10.1126/...

My Site About Me I am currently an Associate Professor in the Department of Human Health and Nutritional Sciences (HHNS) in the College of Biological Sciences (CBS) at the University of Guelph and Director...

The PowerLab ( uofgpowerlab.weebly.com ) is having some big turnover this summer and this opens the door to take on new PhD students to work on projects related to muscular adaptations to use/disuse across various structural levels.

Happy to chat!!

Muscle fiber Myc is dispensable for muscle growth and its forced expression severely perturbs homeostasis - Nature Communications The transcription factor Myc stimulates growth in many cells. This study shows that Myc expression is essential in muscle stem cells to support muscle growth, but is neither sufficient nor necessary f...

www.nature.com/articles/s41...

A human brain map of mitochondrial respiratory capacity and diversity - Nature The ability to physically partition the human brain at a spatial resolution comparable to neuroimaging methods enabled the development of a brain-wide atlas of mitochondrial content, specialization an...

www.nature.com/articles/s41...
A human brain map of mitochondrial respiratory capacity and diversity

In-cell architecture of the mitochondrial respiratory chain Mitochondria regenerate adenosine triphosphate (ATP) through oxidative phosphorylation. This process is carried out by five membrane-bound complexes collectively known as the respiratory chain, workin...

Attention mitochondriacs! Mitochondrial supercomplexes by cryo-electron tomography. How can you not be blown away by how beautiful this is? Fascinating that ATP synthase is largely organized as dimers since this is one of the putative configurations of mPT pore. www.science.org/doi/10.1126/...

SIRT5 safeguards against primate skeletal muscle ageing via desuccinylation of TBK1 - Nature Metabolism SIRT5 protects against primate skeletal muscle ageing through TBK1 desuccinylation, which inhibits the downstream inflammatory and senescent phenotypes.

www.nature.com/articles/s42...

Sedentary death syndrome - PubMed Sedentary death syndrome (SeDS) is a major public health burden due to its causing multiple chronic diseases and millions of premature deaths each year. Despite the impact of physical inactivity, very...

« Use of sedentary subjects as the control group has led to potentially misleading interpretations. If physically active individuals were designated as the control group, a different interpretation would have been drawn ».

21-year-old gem.

pubmed.ncbi.nlm.nih.gov/15317985/

Macrophages excite muscle spindles with glutamate to bolster locomotion - Nature A population of macrophages with exclusive molecular and functional signatures in the muscle spindles express machinery for synthesizing and releasing glutamate, and a cellular component, the muscle s...

Macrophages excite muscle spindles with glutamate to bolster locomotion
www.nature.com/articles/s41...

Serum Proteomic and Metabolomic Signatures of High Versus Low Physical Function in Octogenarians - PubMed Physical function declines with aging, yet there is considerable heterogeneity, with some individuals declining very slowly while others experience accelerated functional decline. To gain insight into...

New article alert from our group! If you have ever wondered what circulating factors associate with extremes of physical function in advanced age in humans, this provides some insights and raises plenty of questions: pubmed.ncbi.nlm.nih.gov/40059508/

Immunomodulatory role of the stem cell circadian clock in muscle repair The stem cell circadian clock orchestrates metabolic and immune signaling to time muscle regeneration following injury.

Immunomodulatory role of the stem cell circadian clock in muscle repair
www.science.org/doi/10.1126/...

Clonal dynamics and somatic evolution of haematopoiesis in mouse - Nature Isolating and studying haematopoietic stem cells in young and aged mice demonstrates evolutionary processes related to blood production and provides a framework for interpreting future work using labo...

Clonal dynamics and somatic evolution of haematopoiesis in mouse

www.nature.com/articles/s41...

Single-nuclei sequencing of skeletal muscle reveals subsynaptic-specific transcripts involved in neuromuscular junction maintenance - Nature Communications Here they use single nuclei RNA-seq to identify transcripts in skeletal muscle that maintain the neuromuscular junction, both under normal and denervated conditions, which allows them to characterize ...

Single-nuclei sequencing of skeletal muscle reveals subsynaptic-specific transcripts involved in neuromuscular junction maintenance

www.nature.com/articles/s41...

Structure of mitochondrial pyruvate carrier and its inhibition mechanism - Nature Structural findings define the architecture of the mitochondrial pyruvate carrier, delineate its substrate-binding site and translocation pathway, and reveal its major conformational states, providing...

Structure of mitochondrial pyruvate carrier and its inhibition mechanism

www.nature.com/articles/s41...

Proximity proteomics reveals a mechanism of fatty acid transfer at lipid droplet-mitochondria- endoplasmic reticulum contact sites - Nature Communications Protein-mediated transport is implicated in trafficking fatty acids at contact sites of lipid droplets and mitochondria. Here, the authors use proteomics to catalogue the proteins at this contact site...

Proximity proteomics reveals a mechanism of fatty acid transfer at lipid droplet-mitochondria- endoplasmic reticulum contact sites

www.nature.com/articles/s41...

Dietary nitrate supplementation mitigates age‐related changes at the neuromuscular junction in mice Abstract figure legend Ageing is associated with a decline in muscle mass and strength, namely sarcopenia, significantly impacting the quality of life in older individuals. Different strategies are b...

physoc.onlinelibrary.wiley.com/doi/full/10....

Dual regulation of mitochondrial fusion by Parkin–PINK1 and OMA1 - Nature We find that, in mice, although the individual loss of Parkin or OMA1 does not affect mitochondrial integrity, their combined loss results in small body size, low locomotor activity, premature death, ...

www.nature.com/articles/s41...

A primer on global molecular responses to exercise in skeletal muscle: Omics in focus Advances in skeletal muscle omics has expanded our understanding of exercise-induced adaptations at the molecular level. Over the past 2 decades, tran…

@DrJimmyBagley and I wrote this while he was fighting (and beating!) cancer. Grateful for him as my colleague and friend. “A primer on global molecular responses to exercise in skeletal muscle: Omics in focus”. Cutting edge! @JSHS_MedHealth #myoblue www.sciencedirect.com/science/arti...

Multicomponent exercises including muscle power training enhance muscle mass, power output, and functional outcomes in institutionalized frail nonagenarians - PubMed This randomized controlled trial examined the effects of multicomponent training on muscle power output, muscle mass, and muscle tissue attenuation; the risk of falls; and functional outcomes in frail...

Very interesting hypothesis Russ! There is some evidence that you can still get an anabolic response, even in nonagenarians, but data on OXPHOS are definitely lacking. We may have to start our own trials!

pubmed.ncbi.nlm.nih.gov/24030238/

🚨APPLICATIONS CLOSE FRIDAY 21st FEBRUARY🚨

For our 2025 Editorial Board Fellowship scheme!

Read about Josiane Broussard's experience as an Editorial Fellow🎓👇

🔗 https://buff.ly/2s5K83y

💪CALL FOR PAPERS💪
For our "New perspectives on the physiological basis of muscle loss" #SpecialIssue! This issue will highlight the latest research underpinning the integrative mechanisms driving muscle loss! Click the link and submit your research!

🔗https://buff.ly/3D97oFr

I may be short sighted though and would love to hear you arguments if you have a different perspective! 5/4.

It could also be an interesting alternative (or a complementary approach) to GLP1 agonists, especially when considering the side effects of physical activity (4/4).