Magda Kopczyńska

Interested in antisense oligonucleotides?
Check our story about downstream-of-gene ASOs (DG-ASOs)!
Together with the Roche Innovation Center we
identified transcription termination windows of protein-coding genes as ASO target regions.

link to BioRxiv:
www.biorxiv.org/content/10.6...

Very excited about this one - it is definitely worth remembering that 3' end processing and transcription termination are distinct processes and play crucial roles in gene expression 🧬

HIRING! STOP lab is looking for a Research Assistant (Technician) with experience in protein expression and purification. Job & project description and how to apply here: euraxess.ec.europa.eu/jobs/367610

Thank you 🙏

And finally:
The effect of SETD2 on transcription is indirect - it likely acts upstream, modulating a plethora of transcription factors. (5/5)

SETD2’s control of readthrough is independent of alternative polyadenylation. It highlights the importance of distinguishing these two processes: 3’ end processing & termination are not the same❗ (4/5)

In cells that don’t have SETD2 or it’s catalytically inactive, readthrough happens because:
1️⃣ TSS initiation is maintained,
2️⃣ 3’ end cleavage is impaired,
3️⃣ cryptic intragenic transcription gets triggered, especially close to the gene ends. (3/5)

We show that SETD2 activity has pleiotropic effects on protein-coding genes:
🟢 promotes proper transcription initiation for most of them (class I),
🛑 stimulates termination in ~20% - prevents transcriptional readthrough (class II). (2/5)

🚨 New preprint from our labs (STOP lab @stoplab.bsky.social & Nojima lab @pol2rna.bsky.social @pyrolyn.bsky.social)! We've been digging into how chromatin remodeler SETD2 controls the start and end of transcription 👇 www.biorxiv.org/cgi/content/... (1/5)

I don't exclude this option :))

Honored to receive an award from @polskaakademianauk.bsky.social in Poznań for the best 2024 publication led by a PhD student. Our study published in @narjournal.bsky.social highlights a key marker defining gene ends — helping improve genome annotations.
Read more 👉 academic.oup.com/nar/article/...

I knew one day I’d have to watch powerful men burn the world down – I just didn’t expect them to be such losers | Rebecca Shaw Elon Musk and Mark Zuckerberg’s desperation to be cool as they suck up to Donald Trump is so cringe it makes my skin crawl

"I have been prepared for evil, for greed, for cruelty, for injustice – but I did not anticipate that the people in power would also be such huge losers." 🤷🏻‍♀️

www.theguardian.com/commentisfre...

Happy to see the first first-author paper from my PhD out! Huge thanks to all co-authors and of course to my brilliant supervisor @stoplab.bsky.social 🙏

Usage of T4ph as a termination marker could benefit more comprehensive genome annotations and help with annotating disease-associated mutations outside of coding regions.

We summarize the findings from literature regarding T4ph and its role in transcription termination. We also provide new analyses that confirm association of T4ph with regions where transcription stops - both at the gene end and prematurely, within the gene body.

Do you know where transcription of a gene actually stops? Hint: it is not the annotated gene end.
In our new paper in Nucleic Acids Research we argue that phosphorylation on threonine 4 of RNA Pol II CTD is a marker of transcription termination in animals... 🧵
academic.oup.com/nar/advance-...

Great news & strategic decision @donald-tusk.bsky.social
@andrzej-domanski.bsky.social: 500 mln top-up for🇵🇱research @ncngovpl.bsky.social in 2025!
Strength. basic research is the best way to drive INNOVATION and INVEST in the FUTURE of 🇵🇱&🇪🇺
A frontline country can do it - what's your gov's excuse?

Coming soon 🫡