It was a Voyager and a very fine instrument . I have a paper with over 6500 citations using that wonderful instrument.
21.03.2025 00:00
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It was a Voyager and a very fine instrument . I have a paper with over 6500 citations using that wonderful instrument.
28 years ago got a DE-STR ( Death Star) and started mass fingerprinting at the MRCPPU in Dundee . All backed up with the wealth of protein chemistry knowledge in Dundee.
However very few can actually show you the raw MS2 spectrum so you can decide if the fragments selected are real or noise. The Swath microApp from Sciex does this for .wiff files and you can filter in or out a lot of interesting hits based on the quality of the MS2 spectrum.
Thanks Bini. That is very useful to know. I think that quantitative proteomics has a way to go but it’s getting there. Need to add internal standards to general workflows though.
That's what I was guessing. In the CRO I was working for before I retired 14 points across the peak was the minimum required which is rarely seen in proteomic experiments. Will be interesting to see how quantitative proteomics evolves and matures.
Astral and TIMS-TOF Ultra users I would be interested in how many points across the peak you are getting from your DIA experiments. If you can share gradient length and points across the peak as I would like to compare with the small mol CRO methods. Quantitative proteomics needs some rigour I think
Just spent a week moving into a new house and totally exhausted. New house not so new though as built in 1576 so I now live in a Elizabethan farm house with very high oak beams. Just back at my desk organising some training and consulting so retirement is busy!!
Love this advert
John doing his best Winston Churchill impersonation .
I would hope the Scottish government will do something to put the Orange one in his place. Ban him from visiting his golf courses in Scotland . That’s how you treat a petulant child.
First official day of retirement. I can highly recommend it. Just waiting for the trout season to open next month.
Enjoy Albert. Fantastic part of the world and a trip to Wye River is worth it for the koalas and king parrots.
Nice thing about being retired is I can come to scotland to see the kids when I want. Also caught up with old colleagues at CRUK Scotland and MRCPPU in Dundee. Now off to Aberdeen.
Business as usual Matt. The orange one huffs and puffs but has to get both houses to approve any of his directives.
0.3mm i.d columns at 5-10ul/min were bulletproof unless it was a failed digest. Usually 2000-5000 injections before needing to be changed.
I have said this for years Luke. Micro is the way ahead and has been for me since 2011.
Lots of respect from me. Too many students and post docs use pre cast without ever knowing the ins and outs of SDS PAGE. Costs plenty too!!
Self poured or pre-cast gels? I remember those days back in the 80's.
In CRO world a minimum of 14 points across the peak is required for accurate quantification. This means your MS has to be fast.
I think we should mix and match. High in Fahrenheit and low in Celsius just to confuse people even more😉
I should really post science discussion here, but this was my son playing #2 Freed Hardmann in the NAIA last night
Thanks Mike. Looking forward to more fishing mixed in with some science.
Less than one month until I retire from full time work. I am going to offer training and consultancy from early march 2025, so if you know of anyone who would like training on LCMS systems or consultancy on proteomics sample prep etc please mention my name.
It is a cool comparison and yes I think Olink is only human proteins. As pointed out there is a conflict of interests from the authors but I think the study is sound and should be discussed. That’s what this place is for.
Nice review of plasma proteomics methods by Josh Coon and his group . However the perchloric acid treatment of plasma will lead to the formation of cysteic acid and methionine sulphone which is not mentioned for the DIA search. .https://www.biorxiv.org/content/10.1101/2025.01.08.632035v1
Standard 2.1mm columns from various vendors are around 700 pounds so price seems about right. If you want a validated column you need to pay I am afraid.
I am doing HILIC of oligonucleotides ( I know!!) and the sodiation I am seeing must be coming from the LC even though it has been thoroughly washed. The ASO made up in ammonium acetate/acetonitrile/milliQ water shows no sign of sodiation when infused. Any ideas on how to reduce the sodiation?
I’m at the end of my career so I can be a grumpy old bugger. Things like this need to be rectified as zi currently work in a GRP company and this is not tolerated at all.
Wouldn't it be refreashing to actually have a method in a paper that describes all the details. I love the reference to a previous paper that tells you absolutely nothing. So common and tolerated in our community.
