Mirian Krystel De Siqueira

Ribosomal RNA (rRNA) genes are found in hundreds of copies in the human genome.

Do sequence variations in these paralogs change the ribosome function? Yes!

I am excited to share our new preprint @mbarnalab @jkpritch in collaboration with Calico:
www.medrxiv.org/content/10.1...

1/8

Dietary control of peripheral adipose storage capacity through membrane lipid remodelling Nature Metabolism, Published online: 27 June 2025; doi:10.1038/s42255-025-01320-yDietary n-6 PUFAs enhance adipose tissue expandability through the PPARγ–LPCAT3 membrane remodelling axis.

ICYMI:

Dietary control of peripheral adipose storage capacity through membrane lipid remodelling - Nature Metabolism Dietary n-6 PUFAs enhance adipose tissue expandability through the PPARγ–LPCAT3 membrane remodelling axis.

Excited to share Chapter #1 of our LPCAT3 journey in adipose biology and membrane dynamics—born of a rewarding, years-long, espresso-fueled partnership with Prof. Yuta Shimanaka @shimachu64.bsky.social. www.nature.com/articles/s42...

Dietary control of peripheral adipose storage capacity through membrane lipid remodelling - Nature Metabolism Dietary n-6 PUFAs enhance adipose tissue expandability through the PPARγ–LPCAT3 membrane remodelling axis.

Congratulations, Marc and Yuta!
www.nature.com/articles/s42...

A cold-inducible phospholipid—protein interaction in brown fat mitochondria optimizes thermogenic capacity Cold stress elicits dynamic remodeling of the mitochondrial lipidome in brown adipose tissue (BAT), marked by an increase in arachidonoyl–phosphatidylethanolamine (AA–PE). However, the function of mem...

Check out the preprint of these two amazing scientists, fat-biologists, and superstar former postdocs Yuta and Marc (BlueSky-less users)!

Super excited to see this story out.

www.biorxiv.org/content/10.1...

Protocol to perform polysome profiling in primary differentiating murine adipocytes Here, we present a protocol for polysome profiling in differentiating adipocytes from the mouse stromal vascular fraction. We describe steps for lysate preparation, ultracentrifugation through sucrose gradients, mRNA isolation, RNA sequencing, and motif enrichment. This protocol enables the analysis of actively translating mRNAs and translational gene regulation by isolating polysome-bound mRNA, revealing insights into protein synthesis during adipogenesis or stimuli responses. Applications incl...

Protocol to perform polysome profiling in primary differentiating murine adipocytes #protocol #starprotocols #cellpress

New protocol drop! 🧪 Check out our polysome profiling in primary differentiating murine adipocytes 🐭🧫 — a step-by-step guide to studying translational control during adipogenesis. 👉 star-protocols.cell.com/protocols/4182
@laurahulea.bsky.social @claudiovillanueva.bsky.social

Grateful to @berkeleyMCB for highlighting our lab and our recent discoveries! It was fun to discuss some of the surprises during our research on lipid homeostasis, lipid droplets, & ferroptosis—driven by exceptional UC Berkeley students & postdocs. mcb.berkeley.edu/news-and-eve...

5/5 In a similar way, Rosi drives the blossom of preadipocytes even in unhealthy environments (obesity)! Cover art by Demin Lu – MolGraphics (www.molgraphics.com)

4/5 Inspired also by the poem from Carlos Drummond de Andrade
“A flower rose from the street!
[…] breaks the asphalt […]
I assure you that a flower rose
Its color is unnoticed
Its petals don’t open
Its name is not in the books […] but it is truly a flower!”

3/5 From this orchestrated bloom, vibrant, mature petals - fat cells in their fullest form - unveil themselves like a masterpiece of adipogenesis.

2/5 These blossoms emerge as a pool of heterogeneous progenitor leaves and preadipocyte buds. Bathed in the essence of Rosiglitazone, the immature petals slowly unfurl, by the crescendo of ribosomal protein expression within the flower’s nuclear heart.

Fat Cells in Full Bloom!
Check out our cover on #CellReports
1/5 In a vibrant homage to Van Gogh's iconic Sunflowers, we introduce the Rosiflowers - a wonder born from the fertile depths of the adipose stromal vascular fraction.

Congratulations Mirian @desiqueiramk.bsky.social , the Villanueva and Yang labs at UCLA! Happy to have contributed to such beautiful work highlighting the role of mRNA translation in adipocyte biology.

5/5: A big thank you to our funding sources for making this research possible @americanheart.bsky.social #UCLA #UCLAIBP - your support is invaluable in advancing our understanding of adipose biology and metabolic health!

4/5: Finally, we show how rosiglitazone treatment enhances translation in adipose progenitors, promoting adipogenesis. Blocking this effect via eIF4A inhibition reverses the benefits, highlighting translation remodeling as a key to adipose tissue health in obesity.

3/5: We discovered that PPARγ-driven ribosomal remodeling enhances adipocyte progenitor and preadipocyte differentiation potential. This effect is directly linked to PPARγ binding to ribosomal genes and is crucial for adipogenesis in obesity.

2/5: Using single-cell RNA sequencing, we mapped how rosiglitazone (a PPARγ agonist) reshapes transcriptional profiles in adipose tissue, shifting obese states to lean-like ones. Key changes include decreased inflammation & increased lipid metabolism and ribosomal activity.

1/5: Adipose tissue is central to energy balance and metabolic health, but obesity disrupts its adaptability. Our latest study in #CellReports explores how translation in adipose progenitors is regulated, revealing key insights into obesity's impact.

PPARγ-dependent remodeling of translational machinery in adipose progenitors is impaired in obesity De Siqueira et al. explore the effects of rosiglitazone on adipose stem cell plasticity, showing that PPARγ stimulates expression of ribosomal factors in adipocyte precursors. This leads to translatio...

Excited to share our latest work in #CellReports highlighting a PPARγ dependent remodeling of translation machinery in adipose progenitors. Amazing collab between our lab Claudio Villanueva, Xia Yang, and @laurahulea.bsky.social !
#obesity #metabolism #translation
www.cell.com/cell-reports...

PPARγ-dependent remodeling of translational machinery in adipose progenitors is impaired in obesity

www.cell.com/cell-reports...

Specific tRNAs promote mRNA decay by recruiting the CCR4-NOT complex to translating ribosomes The CCR4-NOT complex is a major regulator of eukaryotic messenger RNA (mRNA) stability. Slow decoding during translation promotes association of CCR4-NOT with ribosomes, accelerating mRNA degradation....

This is a very cool paper that I will add to my Mol Bio course. Specific tRNA recruitment of mRNA degradation complex to ribosome through the P site tRNA interactions with CCR4-NOT complex 1/ www.science.org/doi/10.1126/...

Join our joint meeting on Obesity & Adipose Tissue / MASH! Submit abstracts for short talks by 11:59 PM MST, December 4th. Visit hubs.la/Q02Z4Jlq0 or hubs.la/Q02Z4G770.
.
.
#KSObesity25 #KSMASH25 #obesity #adipose #adiposetissue #adipocyte #metabolicdisease #MASH #NASH #NAFLD #MAFLD #fibrosis

Are you a scientist working or interested in RNA Translation? Join our starter pack and share ideas, methodologies and tips to advance our knowledge in how life came to be!
#RNA #ribosome

go.bsky.app/VN78eTo

Working on immunometabolism? Or looking for people who do? Then here’s the starter pack for you! 😊 #Immunosky

go.bsky.app/MvxkA4Q

Sharing again as I have added a at least 20 newbies and people how I just found out were here. Just need to get that Giles Yeo over here now go.bsky.app/NRoqMvp