Isaia Vardanega

New Article: "Imputation integrates single-cell and spatial gene expression data to resolve transcriptional networks in barley shoot meristem development" rdcu.be/eYiMF

With Research Briefing rdcu.be/eYiM7

Imputation integrates single-cell and spatial gene expression data to resolve transcriptional networks in barley shoot meristem development - Nature Plants Spatially resolved gene expression during barley development was done by integrating an scRNA-seq dataset from cells with unknown position with spatial transcriptomics. This dataset is publicly availa...

It's finally out!...
Using single-cell and spatial transcriptome data, and data imputation, we created BARVISTA, a website able to perform almost genome-wide information on gene expression profiles at single-cell resolution in barley spikes!
www.nature.com/articles/s41...

Happy to share our recent thoughts on how grasses form and pattern stomata
doi.org/10.1016/j.pb...
🌾From fate establishment, through within-file patterning to dumbbell formation🌾
Congrats to
@lbmountain.bsky.social
on getting two publications out in a single day - a first for the lab!

A wonderful collaboration with our colleagues from Düsseldorf within the @cerealcell.bsky.social consortium. @isaiavardanega.bsky.social @simonrdg.bsky.social Jan Maika.
Thanks to the sequencing facilities at Uni Heidelberg and Uni Bern, and past and present team members for this "tour de force"

I’m proud to present the main work from my postdoc, 3+ years in making from scratch, my ultimate #MimulusPropaganda up till now:

The Moment
That
Symmetry
Breaks.

#plantscience #development #imaging #microscopy 🧪🌸🔬
w. Captain Yaowu & @biancatash.bsky.social

www.biorxiv.org/content/10.1...

@simonrdg.bsky.social

@gwenkirschner.bsky.social

From Tip to Grain: Sculpting Barley Inflorescence Through the Regulation of Meristem Activity - the Node Written by Isaia Vardanega Behind the Paper Story of “CLAVATA signalling shapes barley inflorescence by controlling activity and determinacy of shoot

From Tip to Grain: Sculpting Barley Inflorescence Through the Regulation of Meristem Activity thenode.biologists.com/from-tip-to-...

Meristems shape plant architectures, and grasses generate a complex array of them. To characterise barley vegetative SAMs and spike development, we used single cell and spatial transcriptome data and integrated them in a new database, BARVISTA. A click on a cell now.....
tinyurl.com/38c3mf5d

Camelina plants have been genetically modified to produce omega-3 long chain polyunsaturated fatty acids such as EPA. The photo shows the seed pods of the camelina plant with the structure of EPA superimposed around the seed pod.

#PlantScience Research Weekly: May 23, 2025 plantae.org/plant-scienc... Lost in translation; How to eat an idea; RNA editing in mitochondria; Non-destructive ubiquitin tagging by COP1; When CLV walked in fields of gold (1/2)

Featuring work by @roederlab.bsky.social @rothamsted.bsky.social @isaiavardanega.bsky.social @simonrdg.bsky.social @adhansonlab.bsky.social

Imputation integrates single-cell and spatial gene expression data to resolve transcriptional networks in barley shoot meristem development Grass inflorescences are composite structures, featuring complex sets of meristems as stem cell niches that are initiated in a repetitive manner. Meristems differ in identity and longevity, generate b...

I am thrilled to share our work on integrating single-cell and spatial transcriptomics. Here, we assign a spatial position to protoplasts by gene expression imputation, allowing us to dissect regulatory networks during meristem and organ determination in barley.

www.biorxiv.org/content/10.1...

Excited to see our work finally out! 🌾🏴‍☠️
We show how HvCLV1 and HvFCP1 shape barley inflorescence using innovative techniques and some cool microscopy.
Huge thanks to everyone involved in this study!

t.co/ofButB3fRo

PHOTOPERIOD 1 enhances stress resistance and energy metabolism to promote spike fertility in barley under high ambient temperatures (Tianyu Lan, Agatha Walla, Kumsal Ecem Çolpan Karışan, et. al.) academic.oup.com/plphys/advan... #PlantScience