Johnathan Canton

DNGR-1 signalling limits dendritic cell activation for optimal antigen cross-presentation | The EMBO Journal imageimageDNGR-1 (CLEC9A) is a C-type lectin receptor of type-1 conventional dendritic cells (cDC1s) with an important role in cross-presentation of dead cell antigens. This study explores how it equips cDC1s with the ability to cross-present such ...

Delighted that this study is finally out @embojournal.org. A detailed analysis of how DNGR-1/CLEC9A signals to promote cross-presentation of dead cell-associated antigens yet does not activate dendritic cells.
www.embopress.org/doi/full/10....

@kirstin-rollins.bsky.social, @mmorrissey.bsky.social et al show that #macrophages attack adherent cells using #trogocytosis instead of #phagocytosis. Reducing adhesion increased phagocytosis. Mitotic cells, which disassemble adhesions, are more vulnerable to phagocytosis rupress.org/jcb/article/...

Next week's episode features Dr. Johnathan Canton (@cantonjohnathan.bsky.social) from @ucalgary.bsky.social!

His lab identified a specialized set of proteins enabling cytosolic delivery of engulfed antigens for cross-presentation by #DendriticCells.

Read it in #ScienceImmunology: bit.ly/44ExZEp

HeLa cells stably expressing a dox-inducible active TMEM16F-GFP (blue). Once expressed, lipid scrambling of the plasma membrane bilayer causes vesiculation, shown with Annexin V staining of the exposed PS (gold).

We’ve engineered stable cell systems to induce lipid scrambling of the plasma membrane with active forms of TMEM16F. Scrambling alone causes vesiculation (🔵TMEM16F-active; 🟠PS/Annexin V). Let us know if you want to try the cells out. Preprint: www.biorxiv.org/content/10.1...

Wu et al @sickkidsto.bsky.social show that Snx10 regulates PI(3,5)P2 accumulation on #lysosomes by controlling PI(3)P delivery. Deleting Snx10 inhibits ClC-7, thereby impairing (phago)lysosome resolution. This mechanism may account for Snx10-related #osteopetrosis. rupress.org/jcb/article/...

As usual, a thoughtful and very beautifully illustrated review from the Freeman lab! 🤩

CD47 prevents Rac-mediated phagocytosis through Vav1 dephosphorylation CD47 is expressed by viable cells to protect against phagocytosis. CD47 is recognized by SIRPα, an inhibitory receptor expressed by macrophages and other myeloid cells. Activated SIRPα recruits SHP-1 ...

Wyatt Miller's thesis project is on bioRxiv! We describe the signaling cascade that allows the 'Don't Eat Me' signal CD47 to inhibit phagocytosis. www.biorxiv.org/content/10.1...

Very interesting!

Always a pleasure to discuss and build a review with Nacho Melero & team!!! #immunosky #cancer #cancerimmunology

Cholesterol mobilization regulates dendritic cell maturation and the immunogenic response to cancer - Nature Immunology To mature, dendritic cells collect and synthesize cholesterol to construct lipid nanodomains on their membranes. Obstructing this process impairs their ability to prime T cells.

‘Cholesterol mobilization regulates dendritic cell maturation and the immunogenic response to cancer’

www.nature.com/articles/s41...

Extremely grateful to Damon Runyon for this funding, especially with todays news on NIH grants. See a green macrophage nibbling on a pink cancer cell 👇