Thomas B. Poulsen

Funding: Very very grateful to the @erc.europa.eu (ERC, grant 865738) for the support of this study.

Team: This has been an extraordinary effort from the whole team but especially by co-first authors Florian Kromm, Søren Borcher Møller who overcame countless obstacles along the way. @au.dk @thomastorring.bsky.social

A few highlights:
-11 steps in the longest linear sequence to lasalocid acid A. Five building blocks - four interesting C-C fragment couplings!
-Pivotal Pd/ZnF₂-mediated C-C coupling reaction affords the attached ring product in high yield. Broad substrate compatibility - multiple diastereomers.

Thrilled to share our new paper just published in @acsjacs.bsky.social: a modular, 11‑step total synthesis of lasalocid acid A—enabled by a new Pd‑catalyzed C-glycosylation reaction to construct C(sp³)–C(sp³) attached‑rings.

Read the paper: pubs.acs.org/doi/10.1021/...

Our new paper in Helv. Chim. Acta, the Journal of the Swiss Chemical Society, founded 1917, elaborates on unorthodox reversible Michael addition for cellular uptake; with the groups of @tbpoulsen.bsky.social‬ and @oliverthornseshold.bsky.social‬.

onlinelibrary.wiley.com/doi/10.1002/...

Synthesis and functionalization of vinyl sulfonimidamides and their potential as electrophilic warheads Covalent inhibitor design is dominated by the use of electrophilic acrylamide warheads. One limitation of acrylamides is that there are limited opportunities to modify their electrophilicity, and henc...

Great to see Ivan and Charlie's work on the synthesis and reactivity of vinyl sulfonimidamides out in @chemicalscience.rsc.org Here they show that reactivity either above or below that of the corresponding acrylamide can be achieved. @oxfordchemistry.bsky.social
pubs.rsc.org/en/content/a...

Next up: Thomas Poulsen - diving into precision chemistry from the protein to the proteome! ⚗️ Powerful tools for covalent protein profiling. FANTASTIC work! #ChemBio #Proteomics #CSSB25

Congrats to all of the authors, in particular to Florian Kromm and Søren Borcher Møller who lead this work together. 👏 We are grateful for the @erc.europa.eu opa.eu for funding.
#totalsynthesis

Our new route represents a dramatic shortening compared to classic routes to this compound, which was strongly enabled by development of a new transformation to directly couple the challenging attached oxacyclic rings. We are further excited about the generality of this C(sp3)-C(sp3) coupling method

Delighted to share our newest pre-print. Here, we describe a very short synthesis (11 steps, LLS) of the polyether antibiotic lasalocid acid A.
doi.org/10.26434/che...

18 days left until the #NordicRetrosynthesisChallenge begins...
Is your team ready?
Get ready for the #NordicRetrosynthesisChallenge on 5th of May 2025! For more info check nordicretrosynthesischallenge.com
Sponsored by Carbolution Chemicals GmbH and AstraZeneca
Please share! #chemsky #Chembio

Enjoy the meeting Stephan. Unfortunately, I cannot attend this one but hopefully the tradition continues.

45 days left until the challenge is revealed... Are you familiar with the rules? Get ready for the #NordicRetrosynthesisChallenge on 5th of May 2025! For more info check nordicretrosynthesischallenge.com! Sponsored by Carbolution Chemicals and AstraZeneca
Please share ! #chemsky #Chembio

66 days left until the challenge is revealed... Get ready for the #NordicRetrosynthesisChallenge on 5th of May 2025! For more info check nordicretrosynthesischallenge.com! Sponsored by Carbolution Chemicals and AstraZeneca
Please share and spread the news! #chemsky #Chembio

Do you already have a team to participate in the #NordicRetrosynthesisChallenge on 5th of May 2025?
For more info check nordicretrosynthesischallenge.com!
Sponsored by Carbolution Chemicals and AstraZeneca
Please share and spread the news! #chemsky #Chembio

Check out the summary of the newest paper from our lab. Just out in Chemical Science #chembio #chemicalbiology

Ten simple rules for developing good reading habits during graduate school and beyond

To me, the most important are:
Read often, read broadly (incl. older papers and outside your field), and learn to read some papers in detail and others more superficially (and quickly)

Big Congrats Luca!

#ChemBio

Here is a very interesting new paper from the Baker and Chudasama groups at University College London. A clever example of leveraging reversible disulfide rebridging chemistry to achieve selective lysine modification (via proximity) of antibody-fragments.
pubs.rsc.org/en/content/a...

It is great to have @aekiib.bsky.social from the group of @tbpoulsen.bsky.social as visiting PhD student in the lab. His stay in our lab allows us to exchange a lot of thoughts and technologies between the labs and learn so much from each other. #ChemBio onlinelibrary.wiley....

Cool picture! Very happy about this exchange - Thanks for hosting. So many interesting experiments and data already.

Now that’s a good #ChemSky thread!

Can’t wait to read the final version of this preprint in some top notch journal. Congrats to all involved. Beautiful work 👌🏻

This looks like an interesting application of the oxSTEF probes developed in the Poulsen lab before. Good example of developing interesting chemistry/chemical biology and then asking the right biological questions.

Chemoproteomic mapping of the N-terminal cysteinome The oxidation status of N-terminal cysteines directly dictates protein stability via arginylation and proteasomal degradation. However, only a handful of proteins have been shown to be regulated via t...

Interesting ChemRxiv preprint by the group of @tbpoulsen.bsky.social with first author @esbensv.bsky.social. They used OxSTEF probes to study proteins with N-terminal cysteines in the proteome and used the method to specifically investigate difference in normoxia and hypoxia. doi.org/10.26434/che...

Profiling the proteome-wide selectivity of diverse electrophiles Targeted covalent inhibitors are powerful entities in drug discovery, but their application has so far mainly been limited to addressing cysteine residues. The development of cysteine-directed covalen...

Great to see them apply the proteome-wide profiling method for electrophile reactivity and selectivity that we developed together with @nesvilab.bsky.social in Fragpipe in this exciting system. chemrxiv.org/engage/chemr...

Thanks a lot!

Interested in covalent probes? Check out our most recent pre-print on chemoproteomic profiling of N-terminal cysteines. Nice overview/summary from lead author @esbensv.bsky.social

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The Acetonitrile Olympics

Looking back on the Acetonitrile Olympics. You remember those, right?