A super detailed protocol + video on Cryo-ExM - Cryo-Expansion Microscopy, led by the labs of our former postdocs @marinelap.bsky.social & @ebertiaux.bsky.social.
Clear, practical, and very useful for anyone doing nanoscale imaging 🚀 app.jove.com/t/68595/expa...
As a Christmas present, our Jove paper with @ebertiaux.bsky.social is out and available for everyone ! Check this out & thanks @centriolelab.bsky.social for the nice highlight
C2CD3 localizes to a ring structure observed in the lumen of the distal centriole by in situ cryo-electron tomography. Top: Confocal image of an expanded mouse photoreceptor cell immunolabeled for tubulin (magenta) and C2CD3 (green). DC, daughter centriole; BB, basal body; TZ, transition zone; CC, connecting cilium. Scale bar: 500 nm. Middle: In situ cryo-tomogram slices taken along the longitudinal axis of the centriole and connecting cilium. White arrowheads indicate microtubule triplets, black arrowheads indicate microtubule doublets, orange arrowheads mark the luminal ring structure, and yellow highlights the membrane. Scale bar: 50 nm. Bottom: Model of the architecture of the distal region of the human centriole.
How does C2CD3 contribute to distal appendage formation in centrioles? @ebertiaux.bsky.social @centriolelab.bsky.social show that C2CD3 architecturally organizes the distal #centriole, bridging the luminal distal ring complex & peripheral appendage sites @plosbiology.org 🧪 plos.io/496XZuH
Happy to share our fun team-up with @centriolelab.bsky.social & @stearnslab.bsky.social to reveal the architecture of the mammalian centriole’s Distal Ring 🛟. Very cool multiscale integration of #UExM 📏🔬with #CryoET ❄️🔬. Congrats to @ebertiaux.bsky.social & @computingcaitie.bsky.social #TeamTomo 🧪
🥳 Our new paper on C2CD3 is out in @plosbiology.org
With the labs of @cellarchlab.com , @chgenoud.bsky.social , and @stearnslab.bsky.social , we revealed the mammalian centriole Distal Ring architecture by combining #UExM and in situ #CryoET.
Big congrats to @ebertiaux.bsky.social and all involved.
Thrilled to share that my postdoc paper has just been a published in @plosbiology.org !
Thank you to all co-authors, and a special thanks to Paul and Virginie @centriolelab.bsky.social for their support throughout this work.
Proud of what we achieved together!
journals.plos.org/plosbiology/...
Congrats on the publication!!!!
Our latest T7SS study is now out in Science Advances!
We solved the cryoEM structure of the T7SSb core unit (T7bCU) composed of YukB, YukC, and YukD from Bacillus subtilis, revealing how these components assemble within the secretion machinery.
www.science.org/doi/10.1126/...
Thanks @bioarm.bsky.social :)
Thanks @fronzeslab.bsky.social
Merci
Honored to receive the FEBS Booster Funds 2025.
My sincere thanks to FEBS for this recognition and their support of early-career researchers.
This funding will greatly help advance my work.
Congratulations as well to all fellow 2025 awardees.
We hire!
The CytoMorpho Lab is looking for an engineer with a background in cell and molecular biology to join our team in Paris. This is a 18-month contract position, but we're open to exploring long-term opportunities.
More details here:
cytomorpholab.com/index.php/jo...
A Trypanosoma brucei cell visualized by confocal microscopy after ultrastructure expansion. The cell was immunolabelled for the BILBO1 protein (yellow) and stained with fluorescent NHS-ester (grey). The flagellar pocket is highlighted in pink. Credit: Marie Zelená
The flagellar pocket collar (FPC) is a cytoskeletal structure essential for nutrient uptake & immune evasion in #Trypanosome. @mbonhivers.bsky.social &co use U-ExM to provide novel insights into FPC biogenesis, and reveal 2 unknown cytoskeletal structures @plosbiology.org 🧪 plos.io/4n3bWi6
Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
Be ready for an exciting meeting!
🚨 New EMBO Practical Course!
Ultrastructure Expansion Microscopy: From Cells to Tissue 🔬
📅 20–24 Apr 2026 | 📍 EMBL Heidelberg
Co-organised with @banterlegroup.bsky.social @gautamdey.bsky.social
💡 Register your interest to get notified when registration opens:
🔗 www.embl.org/about/info/c...
💼 PostDoc position (2 years) on Trypanosoma brucei | Bordeaux, France | Lab MFP with Dr. Frédéric Bringaud @univbordeaux.bsky.social @cnrsaquitaine.bsky.social
🔬 Glycerol and differentiation in Trypanosoma brucei
🗓️ Deadline: September 15.
Start: by November 2025
labex-parafrap.fr/fr/emplois/4...
On Friday, Daniel left us after more than 4 happy years in the lab @pasteur.fr and a great paper published today in @jcellsci.bsky.social as well as other contributions!
Many thanks for everything!
journals.biologists.com/jcs/article-...
#Trypanosoma, fixed, expanded, and imaged with #STED, stained for tubulin. Scale bar refers to expanded specimen, original structures are 3-3x smaller.
Image courtesy: @mbonhivers.bsky.social, MFP, CNRS, Univ. Bordeaux, France
#fluorescencefriday #ExM #STEDmicroscopy
🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
Honoured to have been introduced today by former lab member @ebertiaux.bsky.social at the annual Microbiology day of New Aquitaine. My first talk in a (former) church!
Left: Schematic representation of the glycerol metabolism of PCF trypanosomes. This figure shows glycerol metabolism leading to the excreted end products, succinate and acetate in the glycosomes and the mitochondrion, respectively, while gluconeogenesis (production for G6P from G3P and DHAP) is represented in red. The oxidative and non-oxidative branches of the pentose-phosphate pathway (PPP) are highlighted in blue and purple, respectively. The three enzymes addressed in this study (PFK, FBPase, and SBPase), possibly responsible for production of F6P from F1,6BP, are boxed. Right: Images show the glycosomal (top) and cytosolic (bottom) localization of MaLionR-Myc-GPDH and MaLionG by immunofluorescence assays on the OEMaLionR-Myc-GPDH.i and OEMaLionG cell lines, respectively, by using an anti-aldolase immune serum as a glycosomal marker.
#Phosphofructokinase (PFK) is considered an irreversible glycolytic #enzyme. This study shows that #Trypanosomes are the only known organism to use PFK in the gluconeogenic direction under normal conditions, likely due to its compartmentalization within glycosomes @plosbiology.org 🧪 plos.io/43r84Qc
Huge congratulations to @marinelap.bsky.social for receiving the 2025 Young Researcher Award! 🏆👏 sbcf.fr/en/newslette...
Brilliant work with unprecedented level of resolution of the trypanosome axoneme!
Assistant prof / MCF post available in ProParaCyto.
March #ImageOfTheMonth goes to M.Bonhivers from MFP lab (UMR 5234).
Trypanosoma brucei cell was extracted in detergent, expanded 4.2 fold using U-ExM, labelled for the microtubule cytoskeleton (magenta) and for the Hook Complex (yellow), and images usinf scanning confocal microscopy.
A massive thanks to @bladerlab.bsky.social for the opportunity to put this mSphere of influence article together!
Also @dvorinlab.bsky.social, @ebertiaux.bsky.social, Brochet Lab, & @centriolelab.bsky.social for doing science that shows off why parasites are cool!
journals.asm.org/doi/10.1128/...
Thanks to a new mass spec pipeline for analysis of the #tubulin tail, the team of Julia Chamot-Rooke and Mariette Matondo revealed a few surprises for #trypanosome beta-tubulin:
-a new glutamylation site
-far less exhaustive detyrosination
Preprint here:
www.biorxiv.org/content/10.1...
A video clip to see #trypanosomes in #3D #FIB-SEM
Many thanks to Valérie Zeitoun and Jeanne Fenouil for their patience during the recording and to Adeline Mallet and Manu Majrouh at the UBI of @pasteur.fr
www.pasteur.fr/fr/journal-r...
