Thanks to the NIH for funding this research and @tamu.bsky.social TAMU medicine for their constant help and support. (5/5)

These discoveries highlight the remarkable capabilities of MINFLUX for studying cellular transport and other dynamic cellular processes. This work wouldn’t have been possible without the help of Abberior, Sebastian, Ziqiang Huang, Clara, Debolina, @thebioguy.bsky.social , Sandeep Dave (4/5)

Transport pathways reveal pauses and a high viscosity environment. We identified three distinct zones within the central permeability barrier that have different transport and binding characteristics. (3/5)

Here, we have shown overlapping pathways for nuclear import and export, challenging prior models of separate transport routes. Transport events are confined within distinct annular rings of the NPC, with no movement through the center. (2/5)

Overlapping nuclear import and export paths unveiled by two-colour MINFLUX - Nature High spatiotemporal precision tracking using 3D MINFLUX shows that nuclear import and export occur in overlapping regions of the central pore, providing insight into transport across the nuclear pore ...

I’m excited to share our latest research from the Musser Lab, published today in Nature. Using two-color 3D MINFLUX, we directly visualized nuclear import and export events through transport-active nuclear pores. (1/5)
www.nature.com/articles/s41...