Data-Driven AI for Neuroscience Organizers Carsen Stringer, HHMI/Janelia Research Campus Srini Turaga, HHMI/Janelia Research Campus Atika Syeda, HHMI/Janelia Research Campus Fabian Sinz, University of Göttingen Alice Wang,

📢 Applications are open! This meeting brings together scientists using data-driven #AI to analyze + model neural circuits and uncover how the brain performs complex computations. 🧠

Apply by May 28 ➡️ janelia.news/NAI26

@computingnature.bsky.social @sinzlab.bsky.social @srinituraga.bsky.social

Cryo-electron tomography reveals coupled flavivirus replication, budding and maturation - Nature Communications In this study, Dahmane et al use a method called cryo-electron tomography to uncover new details of how tick-borne flaviviruses transform cells into virus factories.

I am super happy to share this paper in its final form. We used FIB milling to "dig in to" cell lines and mouse brains infected with tick-borne flaviviruses, followed by cryo-ET to study the virus replication. It's open access, so have a look!
#virology #teamtomo
www.nature.com/articles/s41...

Scientists identify five ages of the human brain over a lifetime Four major turning points around ages nine, 32, 66 and 83 create five broad eras of neural wiring over the average human lifespan.

www.cam.ac.uk/stories/five...

there was a bit error in 9,32,66,83 should be 7,31,67,83 instead 😁

Atomic resolution cryo-EM at 200 keV Atomic resolution in cryo-electron microscopy was first demonstrated six years ago. This was accomplished using 300 kV electron microscopes equipped with new hardware that provided narrower energy spr...

👀 👀

Atomic-resolution #cryoEM at 200 keV.
www.biorxiv.org/content/10.6...

Structure and organization of AMPA receptor-TARP complexes in the mammalian cerebellum AMPA receptors (AMPARs) are multimodal transducers of glutamatergic signals throughout the brain. Their diversity is exemplified in the cerebellum; at afferent synapses, AMPARs mediate high-frequency ...

Structure and organization of AMPA receptor-TARP complexes in the mammalian cerebellum | Science www.science.org/doi/10.1126/...

🧠🪰 The adult Drosophila brain connectome now gives us a complete wiring diagram of ~140k neurons. But a wiring diagram alone isn’t understanding.

How is this massive network organized?

Our paper tackles that question by mapping community structure across the entire fly brain. 1/

Here is why friends don't let friends calculate FSC on denoised half-maps. I took EMD-17961, denoised the maps using Warp's Noise2Map, and calculated the FSC between various things. With noise gone from the half-maps, their consistency is no longer related to SSNR. Denoised map vs. model still works

🔬 Just out in @NatureComms!
Using cryo-CLEM and cryo-ET, we reveal the unique ultrastructure of dopaminergic varicosities in the striatum.
Amazing collaboration with @davip-bdx.bsky.social and Etienne Herzog @iins-bordeaux.bsky.social
Congratulations to Paul and Robin for this fantastic work! 🎉

Method of the Year 2025: electron microscopy-based connectomics - Nature Methods A large network of interconnected neurons serves as the basis of brain function and of behavior. Methodological advances have enabled the reconstruction of large-scale and even whole-brain connectomes...

Connectomics is method of the year 2025:
www.nature.com/articles/s41...

We tend to think of neurons as either excitatory or inhibitory, but some neurons chemically inhibit their downstream targets while electrically exciting their neighbors. What is gained by having an inhibitory neuron excite its neighbor?

www.biorxiv.org/content/10.6...

Nondestructive X-ray tomography of brain tissue ultrastructure - Nature Methods Optimizations of X-ray nanotomography including the choice of resin allows high-resolution imaging of mouse brain tissue, approaching the resolution of volume electron microscopy. Since it does not re...

An optimized X-ray nanotomography method offers an attractive alternative to volume EM for connectomics. @crick.ac.uk @psich.bsky.social psich.bsky.social @adrianawanner.bsky.social @andreas-t-schaefer.bsky.social @ucl.ac.uk @uclnpp.bsky.social
www.nature.com/articles/s41...

Happy to share the first paper from my journey at @psich.bsky.social towards X-ray connectomics, now out in @natmethods.nature.com: www.nature.com/articles/s41...

Help choose JCB’s cover of the year! Explore 12 breathtaking images submitted by our talented authors and vote for your favorite. Voting closes on Wed. December 31. Don’t miss your chance to have your say in celebrating the beauty of science 👉 www.surveymonkey.com/r/JCB2025Cov...

#CellBio2025

#paper!
X-rays can resolve #ultrastructure in tissues non-destructively.

www.nature.com/articles/s41...

Shoutout to key collaborators at @crick.ac.uk and @psich.bsky.social Ana Diaz, @adrianawanner.bsky.social and @andreas-t-schaefer.bsky.social, and to the whole team that made this possible.

Very excited to continue our amazing collaboration with @apacureanu.bsky.social at @esrf.fr - thank you @wellcometrust.bsky.social and all the collaborators at @crick.ac.uk and @esrf.fr

“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...

“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science www.science.org/doi/10.1126/...

Young Swiss Tomo Organizers Elda, Zanetta, Leonie and Florent

Kuppelraum, University of Bern

David Kalbermatter from Bern

Jean Daraspe from Lausanne

Last Friday (17th), we had an amazing first Young Swiss Tomo meeting in Bern, organized with Elda Bauda, Zanetta Kechagia, and @leonieanton.bsky.social!
Over 70 people from 🇨🇭 gathered to talk about cryo-electron tomography 🔬.
Huge thanks to the DCI for making the event possible! See you next year! 👋

Excited to share our preprint on cytoskeletal organization in astrocytes!
biorxiv.org/content/10.110…

Postdoc @mewynne759 looked at microtubules, IFs/GFAP & actin along long elaborate processes.

Wonderful to collaborate w postdoc @dharshinigopal & @NogalesLab on cryo-ET 🤓

1/x

The connectome of the entire male Drosophila central nervous system is out!! Acquired using FIB-SEM @ 8x8x8nm, we aligned 7.5 mio 2D images aligned into one seamless 3D volume, which was used to segment & proofread neurons and synapses. @hhmijanelia.bsky.social

www.biorxiv.org/content/10.1...

Genetically encoded FerriTag as a specific label for cryo-electron tomography Wang et al. present an optimized labeling strategy for cryo-electron tomography based on genetically encoded FerriTag. This system uses rapamycin to recruit iron-loaded ferritin to target proteins, en...

Paper alert🚨
Label your protein of interest and localize it in cryoET 👁️❄️

Genetically encoded FerriTag as a specific label for cryo-electron tomography: Structure www.cell.com/structure/fu...

Excited to share our latest collaborative work from Erin Schuman's lab @mpibrain.bsky.social : An integrated transcriptomic and proteomic map of the mouse hippocampus at synaptic resolution, now published in @natcomms.nature.com. Here’s a thread summary 🧵
doi.org/10.1038/s414...

The small GTPase Ran defines nuclear pore complex asymmetry Nuclear pore complexes (NPCs) bridge across the nuclear envelope and mediate nucleocytoplasmic exchange. They consist of hundreds of nucleoporin build…

My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...!

Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more!

Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo

Brain Surfaces of 70 primate species

1
To predict the behaviour of a primate, would you rather base your guess on a closely related species or one with a similar brain shape? We looked at brains & behaviours of 70 species, you’ll be surprised!

🧵Thread on our new preprint with @r3rt0.bsky.social , doi.org/10.1101/2025...

Representational drift without synaptic plasticity Neural computations support stable behavior despite relying on many dynamically changing biological processes. One such process is representational drift (RD), in which neurons' responses change over ...

When neurons change, but behavior doesn’t: Excitability changes driving representational drift

New preprint of work with Christian Machens: www.biorxiv.org/content/10.1...

In a new study, @victoriazhanqi.bsky.social and the Ponce Lab reveal that neurons across the primate visual cortex are tuned not just to objects, but to animal features—highlighting a surprising bias in how the brain encodes natural scenes. brain.harvard.edu/hbi_news/whe...

Tau filaments with the Alzheimer fold in human MAPT mutants V337M and R406W - Nature Structural & Molecular Biology Qi et al. used cryo-electron microscopy to determine the structures of tau filaments from the brains of individuals with MAPT mutants V337M and R406W, known to give rise to frontotemporal dementias. T...

Finally, the paper by @qcxy8886.bsky.social on #cryoEM of tau mutants V337M and R406W is out in NSMB (which is not the fastest journal). Tau filaments with these mutations have the same structures as in AD, which may be useful for the development of faster models.

www.nature.com/articles/s41...

Today was a memorable day.. saying goodbye to our trusty Tecnai 12 Spirit! >180,000 images from just Ian and myself...not counting the numerous MSc's PhD's and publications!

End of an era!

Thank you @thermofishersci.bsky.social

In collaboration with the Posthaus Lab, we identify ANTXR2—also the anthrax toxin receptor—as the receptor for Clostridium perfringens NetF pore forming toxin. Cryo-EM shows extensive contact between the pore and the receptor
🧬🧊🔬 #Microbiology #CryoEM #PoreFormingToxins
doi.org/10.1101/2025...

Happy about our new paper in Nature rdcu.be/eukUB
The GluA3 structure provides a framework for understanding this disease-prone glutamate receptor - novel interfaces will provide targets for modulator development

A vector calculus for neural computation in the cerebellum Null space theory predicts that neurons generate spikes not only to produce behavior but also to prevent the undesirable effect of other neurons on behavior. In this work, we show that this competitiv...

Neuronal computation in the cerebellum via a vector calculus.

Work of Mohammad Amin Fakharian, Alden Shoup, Paul Hage, and Hisham Elseweifi

www.science.org/doi/10.1126/...