Pseudomonas Genome Database

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Pseudomonas aeruginosa: ecology, evolution, pathogenesis and antimicrobial susceptibility - Nature Reviews Microbiology Pseudomonas aeruginosa is a functionally versatile bacterium, a leading opportunistic human pathogen and a model organism in microbiology. In this Review, Letizia, Diggle and Whiteley discuss P. aerug...

New review on the ecological and pathogenic versatility of P. aeruginosa:

www.nature.com/articles/s41...

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Are you a #Pseudomonas researcher? Please take our survey and help shape the direction and priority of future pseudomonas.com database development: forms.gle/gTPT6ruf3mqb...
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Smlt1473 inhibits the mucoid phenotype of P. aeruginosa, but data suggest most effective enzyme concentration varies by isolate. (A) Representative image of phenotypic changes for P. aeruginosa UVA 44618 in the presence and absence of Smlt1473 indicating an inhibitory function. (B–F) Each isolate of P. aeruginosa was grown in the presence and absence of Smlt1473, plate contents were collected, and uronic acid concentration, which corresponds to alginate content, was quantified. Uronic acid concentration was determined by measuring the absorbance at 530 nm (A530) of the resulting solution. High A530 corresponds to high alginate content, whereas low A530 corresponds to low alginate content. Y222F is the catalytically inactive form of Smlt1473 used to show that results are due to an active enzyme. The results are means and standard deviations and statistical analysis was performed using a One-way Welch’s ANOVA and Dunnett’s T3 multiple comparison post-hoc test.

A polysaccharide lyase from Stenotrophomonas maltophilia disrupts production of alginate—a dominant component of Pseudomonas aeruginosa biofilms—by P. aeruginosa clinical isolates. The lyase may be useful in infection treatment. Learn more in #AppEnvMicro: journals.asm.org/doi/10.1128/... #MicroSky

Structure of the Pseudomonas aeruginosa PAO1 Type IV pilus
Finally out in PLOS Pathogens. Congrats Hannah Ochner and co-authors. @mrclmb.bsky.social

journals.plos.org/plospathogen...

RNA-seq reproducibility of Pseudomonas aeruginosa in laboratory models of cystic fibrosis | Microbiology Spectrum RNA sequencing (RNA-seq) has revolutionized biology, but many steps in RNA-seq workflows can introduce variance, potentially compromising reproducibility. While reproducibility in RNA-seq has been tho...

Different approaches to preparing and sequencing bacterial RNA libraries capture comparable transcriptional profiles:
journals.asm.org/doi/10.1128/...

#microsky #PsWatch #bacteria

Great to see you here Alain @fillouxlab.bsky.social. Follow him for all things Pseudomonas aeruginosa, biofilms and T6SS

[FSE Bicentenary PhD] Generative AI approaches for synthetic phage genome engineering at The University of Manchester on FindAPhD.com PhD Project - [FSE Bicentenary PhD] Generative AI approaches for synthetic phage genome engineering at The University of Manchester, listed on FindAPhD.com

2 exciting PhD opportunities:
- with @fionawhelan.bsky.social understanding evolution of transmissibility in Pseudomonas www.findaphd.com/phds/project...
- with @claudiaigler.bsky.social Patrick Cai on AI-powered phage genome design www.findaphd.com/phds/project...

Please spread the word!

The Pseudomonas Genome Database is now on Bluesky with the Pseudomonas.com web address! We encourage using the #pseudomonas hashtag to note interesting research #pseudomonas #bacteria #microsky #idsky #PsWatch

How to stress out a Pseudomonas aeruginosa quorum sensing researcher #Microsky

Quorum sensing regulation of Psl polysaccharide production by Pseudomonas aeruginosa | Journal of Bacteriology Pseudomonas aeruginosa biofilms are responsible for many treatment-resistant infections in humans. Many cooperative behaviors in P. aeruginosa are controlled by quorum sensing, but evidence for a dire...

Las and Rhl quorum-sensing systems have distinct roles in regulating production of the matrix polysaccharide Psl: journals.asm.org/doi/10.1128/...

Welcome to our new followers! Looking forward to seeing more of our colleagues here on BlueSky soon.

Mixed strain pathogen populations accelerate the evolution of antibiotic resistance in patients - Na... Here, Caballero et al. provide an in depth characterisation of patients colonized with single or mixed strains of Pseudomonas aeruginosa to demonstrate the impact of within-host diversity on the devel...

Like a keen bean, sharing our latest work on this new app 😎 many many Pseudomonas samples and 4 years later… our paper is out 🎉

TLDR; The diversity of Pseudomonas in ICU patient lungs can accelerate the evolution of antibiotic resistance 🦠

Linking evolutionary biology with clinical outcomes.

Pseudomonas Genome Database version 22.1 has been released. More details at: pseudomonas.com