Check out our latest preprint from @jnvmartinson.bsky.social and @leosong.bsky.social! 🌟🦠
We found that conjugative plasmids can actively eliminate recipient bacteria that resist plasmid acquisition. 🧵
Plasmids weaponize conjugation to eliminate non-permissive recipients https://www.biorxiv.org/content/10.64898/2026.02.10.705089v1
During my postdoc in @therubinlab.bsky.social, @leosong.bsky.social and I aimed to make CRISPR-associated transposons more efficient for editing bacteria.
Couldn’t have done it w/o a CAST of characters at IGI and beyond @innovativegenomics.bsky.social, @cresslab.bsky.social, @doudna-lab.bsky.social
Our identification of homologous recombination effectors RecD and RecA guided coupling λ-Red recombineering with CAST, boosting editing efficiency in model (E. coli) and non-model (P. putida, K. michiganesis) Gammaprotebacteria, expanding CAST’s utility as a precise bacterial genome editor. (2/2)
Fig. 5. Phylogenetic distribution of VchCAST activator and inhibitor genes. The phylogenetic distribution of 11 E. coli regulatory genes was mapped across 80,789 representative bacterial genomes from 92 phyla in Genome Taxonomy Database, release 214.0 (GTDB version 214.0) (72, 73), with at least 10 members. Homologs were identified using AnnoTree (60) and confirmed with the eggNOG database (70).
New in Science Magazine Science Advances from the Doudna Lab, Rubin Lab and Cress Lab —Identification of proteins influencing #CRISPR-associated #transposases for enhanced #GenomeEditing. Read here: https://ow.ly/U0s450XS82n
Happy New Year from the Rubin Lab! 🎇
We're excited to start 2026 off with a fresh publication at @science.org Advances. Here, we identified a cast (😉) of host factors affecting VchCAST using genome-wide screens and validated their improvement or inhibition of CAST activity. (1/2)
Grad school panel event for the @innovativegenomics.bsky.social undergrad and tech journal club!
Congratulations Amanda! Excited to see you and your lab shine at URI and in marine microbiology! 🌊✨
