@mjprigge
Plant biologist at UCSD (how auxin regulates transcription across land plants, from receptors to pol II and the steps in between). Mostly, moss⬌Arabidopsis. Grew up in Minnesota, attended/worked at UMinn, UO, UM, and IU.
I cold-emailed Ralph as a second-year grad student: I wanted to work on moss but had no idea what I was doing. He invited me to spend weeks in his lab, joined my committee, and supported me throughout my PhD - my thesis project would 100% not have been possible without his generosity and kindness
Ralph was incredibly generous when I wanted to start working on moss. He invited me to visit his lab for a day and talk to/shadow people in his lab.
Tuned in to the Czechia v Korea baseball game from the Tokyodome, and saw a UCSD grad hit a home run.
1/ ✨ New preprint alert ✨
It is my pleasure to share:
"Stepwise evolution of the developmental and symbiotic functions of DELLA in land plants"
Here we investigated the role of the single GRAS transcription factor DELLA in #MyMarchantia
doi.org/10.64898/202...
#PlantScience
A thread...
This is over a week old
One of THE BEST rhododendron songs, in my opinion, and this live version is - chefs kiss 😘 - outta sight!
The lyrics speak to the big mysteries of rhododendron and give a sense as to why the plants have been so special to humans for millennia
Im delighted they chose it for their live album!
How should I keep spores of Marchantia polymorpha subsp. ruderalis? Although I have kept them at -30˚C for 1-2 months, they don't germinate so far.
No, I haven’t checked
One of its columns seems to bind the pigmented poly-something of moss fairly irreversibly. Last time, I got nervous and added an extra 1/5-volume wash before eluting. With that, I got all 48 samples’ 260/280 ratios 2.17 ±0.02 and 260/230 ratios 2.38±0.03. (That’s the full range, not SE.)
I have a hunch it would work. The only time I tried was when I purified RNA from a combined sample with half gametophores and half protonemata. (I needed to RT-PCR/clone some 6-7.5 kb cDNAs and wanted a mix of cell types.)
I like Zymo’s plant RNA kit because it’s set up for bead beaters. All 48 preps’ abs ratios were within 2% last time, if I remember correctly. (We generally use treated/untreated protonemata, not gametophores though)
Would it really be a superior deal if it triggers a mass exodus of HBO subscribers? The only show I'd really miss would be John Oliver's, and I can't see it being allowed to continue as is with those owners.
After some introspection, perhaps my negative feelings arose after I was fooled by it right as I started thinking that maybe I could consider myself a botanist.
Thanks for this. I always thought of them negatively, as Zamia cosplayers.
Our lab has one that was handed from person to person for ~15 years. Now, I'm the only person left in the lab, and I don't want it. I remember to water it every month or so and it refuses to wilt. (Anyone want it?)
Please check out:
Functional characterisation of Target of Rapamycin (TOR) signalling in #Physcomitrella
We found a remarkable evolutionary conservation of this central #signalling hub, but also specific differences, esp. in RAPTOR.
link.springer.com/article/10.1...
Great method development!
tRNA-based polycistronic CRISPR/Cas9 system boosts efficiency of multi-gene deletion in the #moss #Physcomitrella
link.springer.com/article/10.1...
Delighted to share our new preprint: "Evolution of moss leaf-like organs through variations in deeply conserved developmental principles."
Led by Ph.D. student Wenye Lin from my lab @irbv.bsky.social ,
in collab. with Yoan Coudert (ENS Lyon) and Richard Smith (JIC).
www.biorxiv.org/content/10.6...
Insightful new papers on #auxin, brilliantly compiled by @jillharrison.bsky.social.
Evolution and development: What makes a merry stem?
www.cell.com/current-biol...
My great-great-great grandpa met a woman in Chicago when his German ship was being repaired in NY (after running ammo to Union troops during the Civil War). He visited her 3 years later ... and met his daughter. The FIL with his shotgun is not visible in their wedding photo.
Figure 1
Cytokinin signaling mediated by KMD2 promotes Arabidopsis hypocotyl grafting success #micropublication #biology #data #caltechlibrary #Arabidopsis #Methods #UndergraduateResearchExperience #NewFinding https://micropublication.org/journals/biology/micropub-biology-001768
I’d seen it several times from different angles before I realized she went after him
Unfortunately, I believe the baseball Minnesota Twins were the most glaring absence from the statement condemning the violence after the latest killing.
Not sure how I missed your question. Retrogen offered it, but today announced that they have shut down operations. After a decade, we need to find a new company for Sanger sequencing.
Probably this bsky.app/profile/bbko...
Characterization of Physcomitium patens Ppb-gata mutant phenotypes.
B‐GATA factors are required for nitrogen‐responsive growth in Physcomitrium patens and Arabidopsis thaliana
Zappone et al.
nph.onlinelibrary.wiley.com/doi/10.1111/...
Hmmm, in the same week one company posted their fastest turnaround on my whole-plasmid sequencing (4h 33m), a local company started offering it for one-third their price ($4.99/plasmid ≤10kb).
An in planta single-cell screen to accelerate functional genetics--very cool application of the TMV vector pTRBO by Elizabeth Sattely’s Lab 😎
www.biorxiv.org/content/10.1...
Great project and super nice working environment 👇
View of US Bank Stadium from the Minnesota Viking’s end zone at the end of the third quarter
Made it to my second NFL game