Latest posts tagged with #365DaysOfScience on Bluesky
SLIC is my go-to cloning and site-directed mutagenesis protocol. It starts with a conventional PCR, so this guide and calculations spreadsheet has it all. (As well as links to more resources). Hope it helps! bit.ly/SLICworkflow #365DaysOfScience
I process information best by making infographics, so here are some more. Hopefully the last MS ones for a bit. They’re all on this blog page with much more information & links to other posts. Hope they can help someone other than just me! #365DaysOfScience thebumblingbiochemist.com/365-days-of-...
Mass spectrometry has sooooo many terms. But here's a bumbling biochemist guide to some of what's inside . . . #365DaysOfScience thebumblingbiochemist.com/365-days-of-...
My thesis and other important mementos of milestones from my journey I found while packing… Huge thanks to all those who have helped me along my journey! Can't wait to put these in my new office next month! #365DaysOfScience
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When it comes to mass spectrometry, don’t confuse high resolution and accurate mass! Also, be careful discussing “accurate mass” as it’s often used synonymously with “exact mass,” but they’re not the same... #365DaysOfScience thebumblingbiochemist.com/365-days-of-...
A mostly free day at CSHL Metabolomics – time to try to organize, consolidate, and cement all this new knowledge before it’s lost (and while I still have time to ask questions!) #365DaysofScience
Yes, you should still learn to code! With AI, you can now do more than ever with a basic proficiency. So, in a way, it’s more important than ever! So, in a way, it’s more important than ever! youtube.com/shorts/Mml-n... #365DaysOfScience bit.ly/why_program
A practical guide to FASTA format for protein & nucleic acid sequences & UniProt FASTA headers youtu.be/zuS36HLaZg4 #365DaysOfScience
Science behind the headlines: First patient treated with custom CRISPR (to correct a metabolic disorder called CPS1 deficiency) - A deep dive, journal-club style youtu.be/1pSInDZ3rN4 thebumblingbiochemist.com/365-days-of-... #365DaysOfScience
Moving labs means cleaning up messes. So let’s talk autoclaves! Laboratory autoclaves: a practical guide to what, why, and how youtu.be/Iq2yEyXMoNs #365DaysOfScience #chemsky #realtimechem
An overview of PDB (Protein Data Bank) entries: What's where & how to get structural info you need
YouTube: youtu.be/5wMwlsChz98
MUCH more here: bit.ly/pdbstructures & bit.ly/thepdb
#365DaysOfScience
Continuing the IR testing saga! Yesterday, the results from the war of the drying methods! #365DaysOfScience youtu.be/fEBY7mkQntc
It’s the end of an era as I start to transition to a new one. Today was Nicholas, Haley, and my last day of wetwork in the lab at SMC. Now, it’s just data analysis, presentations, and lab clean up… #365DaysOfScience youtu.be/TeyhlKay3Xc
Continuing the IR testing saga! Today, war of the drying methods. We need our samples to be water-less so we’re comparing lyophilization (freeze drying) and air drying into a sort of film…Tomorrow, we’ll compare methods and see which worked best! #365DaysOfScience
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How determine how many turns of the TCA you’d need to completely oxidize a TCA cycle intermediate, and what you’d generate in the process. (And a warning that AI is bad at this!) ATP accounting for the TCA: youtu.be/9ttZ1GU6t5c #365DaysOfScience
* Downloadable guides to resources, lab math, and word parts
* Laboratory cheat sheets
. . . And more! So, hope this helps you find what you may be looking for! youtu.be/oPHrBio_dH0 thebumblingbiochemist.com/lets-talk-sc... #365DaysOfScience #chemsky #biochemsky #realtimechem
One of the best things about being a biochemist is getting to use all sorts of techniques and equipment to approach questions from different angles (and frequencies!) Today IR (infrared) spectroscopy to analyze bacterial cell functional groups #365DaysOfScience youtu.be/D0-X4gVqO-k
When it comes to multichannel pipettes, evenness is the name of the game! Apply even, vertical pressure to make sure your liquid levels are the same! #365DaysOfScience #chemsky #biochemsky
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The main reason the heavy metal chromium is so toxic is that it’s an oxidant. The hexavalent form of Cr, Cr(VI) can attack and oxidize things, which is bad in our bodies, but allows us to detect its presence using a DPC (1,5-diphenylcarbazide) assay. youtu.be/LQfpBIuWUxs #365DaysOfScience #chemsky
If you want to find proteins that are similar in sequence, turn to BLAST (specifically blasts). If you want to find proteins that are similar in structure, turn to Foldseek! More: bit.ly/uniprotprotp... #365DaysOfScience
When I want to find information about a protein(s), my first stop is almost always UniProt. Here's an overview of this amazing tool and how I try to make the most of it. youtu.be/f75f6QCe1gA bit.ly/uniprotprotp... #365DaysOfScience #chemsky #biochemsky
Today we’re generating calibration curves relating OD600 to dry bacterial mass. Why? In order to "normalize" data from different bacterial samples by the "amount" of bacteria, we need a better way than just measuring OD600, which is a measure of bacterial growth, but w/limitations #365DaysOfScience
If you’re looking to create and/or visualize (interactively or for figures) protein alignments, I added figures & a video to some of my favorites to my blog & my downloadable guides with links. Hope they help! #365DaysOfScience #chemsky #biochemsky
bit.ly/proteinalign... youtu.be/BesY9nHribI
If you’re looking to create and/or visualize (interactively or for figures) protein alignments, here are a few free resources I recommend... youtu.be/BesY9nHribI #365DaysOfScience #chemsky
If anyone has some leftover Peeps this Easter, might I suggest demonstrating ideal gas laws? bit.ly/peepsidealga... #365DaysOfScience #chemsky
Why’s matzoh flat, but bread is fluffy? Fluffiness comes from gas generation in dough that causes it to expand (leaven), which can come from biological fermenters (yeast) or chemical leavening agents bit.ly/leaveningage... #365DaysOfScience
Since I started burning out in grad school, I’ve made a real effort to be sure to take “me” time when I have days off! It’s way too easy to give in to guilt, but self-care is an important part of being a scientist (one I struggle with but tempting cryoEM legos definitely help with) #365DaysOfScience
One of the highlights of our trips was meeting with a collaborator to discuss an upcoming paper. As part of it, I introduced my research students to ORCiD’s . . . bit.ly/quickstartsc... #365DaysOfScience #chemsky
