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Fig. 3. Arrow map of the plasmid pB2126 found in B21128 and B26140 isolates. Antibiotic resistance and other relevant genes were coloured according to the legend presented in the figure.

Fig. 3. Arrow map of the plasmid pB2126 found in B21128 and B26140 isolates. Antibiotic resistance and other relevant genes were coloured according to the legend presented in the figure.

Genomic screening of eight antibiotic-resistant Pseudomonas isolated from rainbow trout (Oncorhynchus mykiss). See more in ACMI: https://doi.org/10.1099/acmi.0.001029.v3

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Fig. 1. Age ranges of studied patients (n=97).

Fig. 1. Age ranges of studied patients (n=97).

Listeriosis – a retrospective study of 5 years on risk factors and clinical outcomes at a tertiary care hospital in Islamabad, Pakistan. Learn more in ACMI: https://doi.org/10.1099/acmi.0.001069.v3
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Sketch map of Lagos lagoons showing some of the sampling locations (software reference: ArcGIS 12).

Sketch map of Lagos lagoons showing some of the sampling locations (software reference: ArcGIS 12).

Assessing the potential for Crude Oil degradation by Biosurfactant-producing Bacteria isolated from Marine Ecosystems in Nigeria. Available to read in ACMI: https://doi.org/10.1099/acmi.0.000953.v6 #AccessMicro

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Logo of Microbiology Society with a stylized blue and green abstract M on a white background.

Logo of Microbiology Society with a stylized blue and green abstract M on a white background.

Detection of aac(3)IIc, aac(6)Ib and armA gene encoding aminoglycoside resistance in Klebsiella pneumoniae in Burkina Faso. Read now in ACMI: https://doi.org/10.1099/acmi.0.001115.v3 #AccessMicro

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Fig. 1: Appearance of L. garvieae isolate on horse blood agar after 24 h incubation at 37 °C in 5% CO2.

Fig. 1: Appearance of L. garvieae isolate on horse blood agar after 24 h incubation at 37 °C in 5% CO2.

Lactococcus garvieae prosthetic aortic and mitral valve endocarditis with multiple embolic complications: a case report. Published Open Access and fee-free in ACMI using a Publish and Read agreement: https://doi.org/10.1099/acmi.0.001149.v3 #AccessMicro #PublishandRead

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Fig. 1.
Illustration of serial dilution technique for MIC testing. Sequential transfer of 2 ml solution creates a concentration gradient across five tubes. Final tubes contain decreasing concentrations labelled as 4x, 2x, 1x, 0.5x and 0 MIC.

Fig. 1. Illustration of serial dilution technique for MIC testing. Sequential transfer of 2 ml solution creates a concentration gradient across five tubes. Final tubes contain decreasing concentrations labelled as 4x, 2x, 1x, 0.5x and 0 MIC.

Developing bacterial resistance to antibiotics: a laboratory activity. Published in ACMI as part of the Pedagogy collection: https://doi.org/10.1099/acmi.0.001118.v3
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Identification of co-transformants. Pyrithiamine-resistant transformants are selected on RM supplemented with pyrithiamine and then purified by streaking for single colonies on AMM supplemented with pyrithiamine. gDNA is then extracted from purified pyrithiamine-resistant transformants and those that have integrated the marker-free construct at the locus of interest are identified by PCR or other genotyping methods. A red circle indicates the expected product size for a co-transformant; on average, 11% of transformants are successfully co-transformed.

Identification of co-transformants. Pyrithiamine-resistant transformants are selected on RM supplemented with pyrithiamine and then purified by streaking for single colonies on AMM supplemented with pyrithiamine. gDNA is then extracted from purified pyrithiamine-resistant transformants and those that have integrated the marker-free construct at the locus of interest are identified by PCR or other genotyping methods. A red circle indicates the expected product size for a co-transformant; on average, 11% of transformants are successfully co-transformed.

Co-transformation of Aspergillus fumigatus: a simple and efficient strategy for gene editing without linking selectable markers. Available to read in ACMI: doi.org/10.1099/acmi...

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Representative epifluorescence micrograph of sterilized compost inoculated with 106 c.f.u. E. coli O157/g and incubated to induce cell elongation. Scale bar represents 10 µm.

Representative epifluorescence micrograph of sterilized compost inoculated with 106 c.f.u. E. coli O157/g and incubated to induce cell elongation. Scale bar represents 10 µm.

Nonculturable Escherichia coli O157 in horticultural compost: a public health concern. Published Open Access and fee-free in ACMI using a Publish and Read agreement: doi.org/10.1099/acmi...

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Two petri dishes each containing growth on SDA plate after 4 days of aerobic incubation at 37 °C (Reverse and obverse) on a yellowish agar medium.

Two petri dishes each containing growth on SDA plate after 4 days of aerobic incubation at 37 °C (Reverse and obverse) on a yellowish agar medium.

Cryptococcus albidus (Naganishia albida) meningitis in a young patient with T-cell acute lymphoblastic leukaemia. Find the full details in ACMI: doi.org/10.1099/acmi... #AccessMicro

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Ultrasound scan of the left shoulder showing subacromial-deltoid bursitis and tenosynovitis of the long biceps tendon

Ultrasound scan of the left shoulder showing subacromial-deltoid bursitis and tenosynovitis of the long biceps tendon

Articular flare-up of systemic scleroderma revealing a rare form of tuberculosis of the shoulder and extensor tendons: a case report and literature review. Read the full report in ACMI: doi.org/10.1099/acmi... #AccessMicro

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Schematic of obesity-associated microbes correlated with inflammasome-related genes (Kruskal–Wallis, P<0.05).

Schematic of obesity-associated microbes correlated with inflammasome-related genes (Kruskal–Wallis, P<0.05).

Obesity-related microbial dysbiosis as a potential modulator of tumour progression. Published Open Access and fee-free in ACMI using a Publish and Read agreement:https://doi.org/10.1099/acmi.0.001055.v4 #AccessMicro #PublishAndRead

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Outstretched hand passing to another hand holding soil with sapling

Outstretched hand passing to another hand holding soil with sapling

It's World Soil Day! Today we highlight the importance of healthy soils in supporting arbuscular mycorrhizal fungi, which enhances plant growth. Find out more in ACMI: doi.org/10.1099/acmi... #AccessMicro

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MRI of the head and spine. (a) Axial T1 post-gadolinium contrast of the brain. Focal leptomeningeal enhancement high left frontal lobe. (b) Axial T2 fluid-attenuated inversion recovery of the brain. A focal high signal lesion at the parafalcine cortex right frontal lobe 

Hear more in the article.

MRI of the head and spine. (a) Axial T1 post-gadolinium contrast of the brain. Focal leptomeningeal enhancement high left frontal lobe. (b) Axial T2 fluid-attenuated inversion recovery of the brain. A focal high signal lesion at the parafalcine cortex right frontal lobe Hear more in the article.

Herpes simplex virus type 2 implicated in a case of acute disseminated encephalomyelitis. Published Open Access and fee-free in ACMI using a Publish and Read agreement: doi.org/10.1099/acmi... #AccessMicro #PublishAndRead

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T2-weighted brain MRI on first admission (a–d) and second admission (e–h). On first admission, a low-density area is noted in the left parietal lobe, right posterior corpus callosum, right occipital lobe, left temporal lobe and cerebellum. After 10 days, the follow-up MRI on second admission shows ventricular enlargement, and periventricular low-density areas were observed predominantly in the inferior horn of the right lateral ventricle.

T2-weighted brain MRI on first admission (a–d) and second admission (e–h). On first admission, a low-density area is noted in the left parietal lobe, right posterior corpus callosum, right occipital lobe, left temporal lobe and cerebellum. After 10 days, the follow-up MRI on second admission shows ventricular enlargement, and periventricular low-density areas were observed predominantly in the inferior horn of the right lateral ventricle.

Balamuthia mandrillaris amoebic encephalitis clinically mimicking myasthenia gravis exacerbation: a diagnostic challenge. Published Open Access and fee-free in ACMI using a Publish and Read agreement: doi.org/10.1099/acmi... #AccessMicro #PublishAndRead

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Percentage distribution of respondents answering each question correctly or incorrectly (Q7–Q13). Respondents (n=1,222) chose ‘True’ or ‘False’ or ‘Unsure’. ‘True’ and ‘False’ responses were converted to correct or incorrect as appropriate.

Percentage distribution of respondents answering each question correctly or incorrectly (Q7–Q13). Respondents (n=1,222) chose ‘True’ or ‘False’ or ‘Unsure’. ‘True’ and ‘False’ responses were converted to correct or incorrect as appropriate.

Assessment of health and science undergraduate students’ knowledge, attitudes, education and training related to antibiotic use and antimicrobial resistance in 27 EU/EEA universities. See more in ACMI: doi.org/10.1099/acmi... #AccessMicro

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Gel photograph showing the amplified products. Plate 1.0. Gel photograph showing the amplified products of the NP1 gene region of HBoV in human respiratory samples. Negative control (N), positive control (P) and 100 bp ladder (L) (100 bp). Lanes 1–7 and 8–13: PCR products for NP1 gene region (lane 3 showing positive result and other lanes showing a negative result).

Gel photograph showing the amplified products. Plate 1.0. Gel photograph showing the amplified products of the NP1 gene region of HBoV in human respiratory samples. Negative control (N), positive control (P) and 100 bp ladder (L) (100 bp). Lanes 1–7 and 8–13: PCR products for NP1 gene region (lane 3 showing positive result and other lanes showing a negative result).

Detection and genetic characterization of human bocavirus 1 infection: evolutionary insights in Western Province Sri Lanka. Read now in ACMI: doi.org/10.1099/acmi... #AccessMicro

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Table displaying distribution of monomicrobial and polymicrobial infections among diabetic and non-diabetic gingivitis patients. Polymicrobial infections were significantly more frequent in diabetic patients than in non-diabetic patients, highlighting the association between diabetes and complex microbial colonization in gingivitis.

Table displaying distribution of monomicrobial and polymicrobial infections among diabetic and non-diabetic gingivitis patients. Polymicrobial infections were significantly more frequent in diabetic patients than in non-diabetic patients, highlighting the association between diabetes and complex microbial colonization in gingivitis.

Prevalence and characterization of β-lactamase-producing bacteria in gingivitis among diabetic and non-diabetic patients: a comparative microbiological study. Find full details in ACMI: doi.org/10.1099/acmi... #AccessMicro

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Post-operative L) hip x-ray following (a) revision and (b) fixation.

Post-operative L) hip x-ray following (a) revision and (b) fixation.

A novel case of prosthetic joint infection due to Clostridioides difficile successfully suppressed with oral doxycycline. Learn more in ACMI: doi.org/10.1099/acmi... #AccessMicro

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Proportion of P. aeruginosa isolates in different antimicrobial resistance categories.

Proportion of P. aeruginosa isolates in different antimicrobial resistance categories.

In vitro activity of cefiderocol against Pseudomonas aeruginosa isolated from adult patients with cystic fibrosis. Published Open Access and fee-free in ACMI using a Publish and Read agreement: doi.org/10.1099/acmi... #AccessMicro #PublishAndRead

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Lateral-flow cassettes are two in number. Each cassette contains one sensitized strip. (a) contains nitrocellulose membrane sensitized with gold NP monoclonal antibody directed against IMP carbapenemase (‘I’ line) and VIM carbapenemase (‘V’ line) and a control capture reagent (‘C’ line). (b) is directed against NDM carbapenemase (‘N’ line), KPC carbapenemases (‘K’ line) and OXA-48-like carbapenemase (‘O’ line) and a control capture reagent (‘C’ line).

Lateral-flow cassettes are two in number. Each cassette contains one sensitized strip. (a) contains nitrocellulose membrane sensitized with gold NP monoclonal antibody directed against IMP carbapenemase (‘I’ line) and VIM carbapenemase (‘V’ line) and a control capture reagent (‘C’ line). (b) is directed against NDM carbapenemase (‘N’ line), KPC carbapenemases (‘K’ line) and OXA-48-like carbapenemase (‘O’ line) and a control capture reagent (‘C’ line).

A prospective study on the evaluation of a novel immunochromatographic card for the rapid detection of five carbapenemase enzymes in metallo-beta-lactamase producers. Available to read in ACMI: doi.org/10.1099/acmi... #AccessMicro

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Intervention period flow diagram.

Intervention period flow diagram.

Diagnostic stewardship – optimization of superficial wound swab cultures can reduce the environmental impact of the microbiology laboratory. Published Open Access and fee-free in ACMI using a Publish and Read agreement: doi.org/10.1099/acmi... #AccessMicro #PublishAndRead

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Erysipelothrix rhusiopathiae-associated bloodstream infection in a patient with systemic lupus erythematosus: a case report and literature review Introduction. Systemic human infections caused by Erysipelothrix rhusiopathiae have been increasingly reported especially within immunocompromised hosts and those with significant occupational…

It's Lupus Awareness Month! A case study highlights the risks faced by those with lupus. Read more in the Open Access journal ACMI: doi.org/10.1099/acmi... #AccessMicro #LupusAwarenessMonth

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(a) Lung with multifocal nodules; (b) H&E-stained images of lung histopathology (600× magnification) showing yeast cells (yellow arrows); (c) fungal colony in the mycelial form growing at 30 °C in inhibitory mould agar; (d) lactophenol aniline blue dye-stained fungal colony; see the lollipop-shaped microconidia arising from the hyphae (400× magnification).

Fig. 1. (a) Lung with multifocal nodules; (b) H&E-stained images of lung histopathology (600× magnification) showing yeast cells (yellow arrows); (c) fungal colony in the mycelial form growing at 30 °C in inhibitory mould agar; (d) lactophenol aniline blue dye-stained fungal colony; see the lollipop-shaped microconidia arising from the hyphae (400× magnification).

Infection caused by a cryptic fungal species, Blastomyces gilchristii, in a tiger. Published Open Access and fee-free in ACMI using a Publish and Read agreement: doi.org/10.1099/acmi...

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Fig. 1.
Results of α-diversity analysis at the species level.

Fig. 1. Results of α-diversity analysis at the species level.

Potential role of gut bacteria in the development of hepatocellular carcinoma. Get the details in ACMI: doi.org/10.1099/acmi... #AccessMicro

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Fig. 3.
NMDS plot showing the visualization results of the random forest model. The centre of the groups (centroids) is labelled with the group name, and the lines indicate how far the samples are from their respective centroids. The centroid for ‘Scalp_S’ cannot be seen as it is underneath ‘Scalp_U’ and that for ‘Axilla_S’ is underneath ‘Axilla_U’.

Fig. 3. NMDS plot showing the visualization results of the random forest model. The centre of the groups (centroids) is labelled with the group name, and the lines indicate how far the samples are from their respective centroids. The centroid for ‘Scalp_S’ cannot be seen as it is underneath ‘Scalp_U’ and that for ‘Axilla_S’ is underneath ‘Axilla_U’.

At-home, self-sampling of the skin microbiome: development of an unsupervised sampling approach. Published Open Access and fee-free in ACMI using a Publish and Read agreement: doi.org/10.1099/acmi... #AccessMicro #PublishAndRead

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Molecular analysis of HBV pre-core gene mutations in patients co-infected with HIV at a tertiary care hospital in North India. Read now in ACMI: doi.org/10.1099/acmi... #AccessMicro

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Standard X-ray of the left leg, frontal (a) and lateral (b) views, showing a marked osteosclerosis (indicated by the red arrow) in the distal tibial region.

Standard X-ray of the left leg, frontal (a) and lateral (b) views, showing a marked osteosclerosis (indicated by the red arrow) in the distal tibial region.

Tibial osteitis caused by Mycobacterium tuberculosis. Read the full case report in Access Microbiology: doi.org/10.1099/acmi... #AccessMicro

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Overview of the classroom experience in an immersive VR environment, integrating new simulations with existing interactive and group-based classroom experiences.

Overview of the classroom experience in an immersive VR environment, integrating new simulations with existing interactive and group-based classroom experiences.

Immersive virtual reality in second-level education: a partnered narrative on the challenges and opportunities for STEM engagement. Get the full details in Access Microbiology: doi.org/10.1099/acmi... #AccessMicro

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Phylogenetic comparison of NZ and global SARG strains. The distribution of global SARG strains, with NZ strains highlighted, is shown on a mid-point rooted maximum-likelihood phylogenetic tree, created by IQ-TREE (1,000 bootstrap replicates) from the Panaroo core gene SNP alignment. The circular format clearly shows the tree structure and strong branch support values.

Phylogenetic comparison of NZ and global SARG strains. The distribution of global SARG strains, with NZ strains highlighted, is shown on a mid-point rooted maximum-likelihood phylogenetic tree, created by IQ-TREE (1,000 bootstrap replicates) from the Panaroo core gene SNP alignment. The circular format clearly shows the tree structure and strong branch support values.

Characterization of Staphylococcus argenteus in Christchurch, New Zealand, and comparison to global strains. Available to read in Access Microbiology: doi.org/10.1099/acmi... #AccessMicro

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Evaluation of the efficacy of cephalosporin antibiotics sold in Kano, Nigeria, against clinical bacterial isolates. Discover more in Access Microbiology: doi.org/10.1099/acmi... #AccessMicro

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