Top left: Primary cells were isolated from brain, lung, kidney, liver, and spleen of a single C. perspicillata bat. Bottom left: CaPsm-K primary and immortalized cells were transfected with 10 μg of poly (I:C) chemically labeled with rhodamine (pIC-Rho) for 16 h. Following fixation, cells were stained with antibodies against GAPDH and DAPI and visualized by confocal microscopy. Scale bars represent 25 μm. Right: Clonal transduced kidney cells infected with Seoul virus (SEOV) were monitored by end-point microscopy for Seoul nucleocapsid protein.
Research on the #bat innate #ImmuneResponse to pathogens is hindered by a lack of lab reagents. @fviromics.bsky.social @stephseifertphd.bsky.social @virology.bsky.social &co generate new bat #CellLines, enabling studies on viral susceptibility & immune responses @plosbiology.org 🧪 plos.io/4jkXukf