Gel from spike and serial dilution experiments. Rows A–D show primer set RAL08, while rows E–H show primer set RAL10. Wells 1 and 49 contain D. purpurea ‘Camelot Lavender’ leaf issue, and wells 2 and 50 contain D. purpurea ‘Alba’ leaf tissue. Wells 3–6 and 51–54 contain D. purpurea ‘Camelot Lavender’ flower, stem, seed pods, and root, and wells 7–10 and 55–58 contain ‘Alba’ flower, stem, seed pods, and root. Wells 11 and 59 are negative controls for the tissue test PCR. Wells 13, 14, 61, and 62 were used to confirm there was no background amplification in 0% biomass Digitalis matrix samples.
From WGS to gels: Development and testing of PCR primers targeting toxic #Digitalis in support of food safety
New in #AppsPlantSci by Hunter, Fo, Literman, Uva, Wolny & Handy
bsapubs.onlinelibrary.wiley.com/doi/full/10.... @fda.gov #foodsafety #botany #plantscience #wholegenomesequencing