#JBacteriol

Ákos T Kovács

@evolvedbiofilm.bsky.social

3 weeks ago

Special Collection focused on Biofilms in #JBacteriol 👇

connected to the 2025 ASM Conference on Biofilms
@asm.org @asmbiofilms2025.bsky.social

Ákos T Kovács

@evolvedbiofilm.bsky.social

3 weeks ago

Bacillus thuringiensis and insects: a century of intimate history

#JBacteriol #Minireview from Leyla Slamti and Didier Lereclus

journals.asm.org/doi/10.1128/...

Ákos T Kovács

@evolvedbiofilm.bsky.social

1 month ago

The Pseudomonas aeruginosa PrrF sRNAs and PqsA promote biofilm formation at body temperature | Journal of Bacteriology Biofilm formation is a critical virulence trait for many microbial pathogens that confers tolerance to the host immune system and antimicrobials. Pseudomonas aeruginosa is an opportunistic pathogen that forms biofilms resulting in treatment failure. Iron is a known requirement for P. aeruginosa biofilm formation, yet the precise role of iron in biofilm physiology remains unclear. Here, we show that temperature alters the requirement for the PrrF small regulatory RNAs, key components of P. aeruginosa’s iron starvation response, for biofilm formation. Specifically, PrrF is required for the optimal formation of flow-cell biofilms at 37°C but not at 25°C, yet most flow-cell biofilm studies are conducted at 25°C. These results demonstrate a previously underappreciated role of temperature in P. aeruginosa biofilm physiology.

The Pseudomonas aeruginosa PrrF sRNAs and PqsA promote biofilm formation at body temperature

-in #JBacteriol

journals.asm.org/doi/10.1128/...

George O'Toole (he/him/his)

@geiselbiofilm.bsky.social

2 months ago

JB Editor's Choice: Caudill and Caswell review the atypical quorum sensing system Brucella species, which have features that distinguish this QS system from typical Gram-negative species.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriol

Ákos T Kovács

@evolvedbiofilm.bsky.social

2 months ago

A 3D-printed capillary tube holder for high-throughput chemotaxis assays | Journal of Bacteriology Chemotaxis is an important behavior to study to understand how microbial communities assemble and respond to their environment. Identifying chemoattractants may uncover key targets for microbiome engi...

A 3D-printed capillary tube holder for high-throughput chemotaxis assays

#JBacteriol

journals.asm.org/doi/10.1128/...

Ákos T Kovács

@evolvedbiofilm.bsky.social

2 months ago

Pseudomonas aeruginosa, my model for research on quorum sensing, biofilms, and opportunistic infections | Journal of Bacteriology With encouragement from the Journal of Bacteriology Editor in Chief, George O’Toole, I have written this short essay in a style different than Journal of Bacteriology articles on model organisms. It i...

Pete Greenberg's view in #JBacteriol:
Pseudomonas aeruginosa, my model for research on quorum sensing, biofilms, and opportunistic infections

journals.asm.org/doi/10.1128/...

Ákos T Kovács

@evolvedbiofilm.bsky.social

3 months ago

Role of FlhF and its domains in the assembly of a polar flagellum in P. aeruginosa | Journal of Bacteriology Pathogenic bacteria rely on flagellar motility for infection and colonization. FlhF, a member of the SRP GTPase family, is a key factor regulating flagellation patterns in various bacteria. In this st...

Role of FlhF and its domains in the assembly of a polar flagellum in P. aeruginosa

#JBacteriol from Deepti Jain

journals.asm.org/doi/10.1128/...

Ákos T Kovács

@evolvedbiofilm.bsky.social

5 months ago

Intracellular glutamine fluctuates with nitrogen availability and regulates Mycobacterium smegmatis biofilm formation | Journal of Bacteriology A subset of nontuberculous mycobacteria (NTM), including Mycobacterium abscessus, are opportunistic pathogens that can cause severe pulmonary infections. Biofilm formation renders M. abscessus more tolerant to antibiotics; hence, the ability to inhibit NTM biofilm formation could help us better prevent and treat NTM infections. However, the regulatory systems controlling NTM biofilm formation, which could include targets for anti-biofilm therapeutics, are poorly understood. The significance of this work is that it reveals intracellular glutamine as an important node controlling the initiation of biofilm formation in the model NTM Mycobacterium smegmatis. Building on this foundation, future studies will investigate how NTM biofilms can be dispersed by altering glutamine levels and will describe how NTM translates intracellular glutamine to the alteration of surface adhesins.

Intracellular glutamine fluctuates with nitrogen availability and regulates Mycobacterium smegmatis biofilm formation

#JBacteriol

journals.asm.org/doi/10.1128/...

Ákos T Kovács

@evolvedbiofilm.bsky.social

5 months ago

Inhibitory proteins of Bacillus subtilis interact within the membrane to block intramembrane protease activity | Journal of Bacteriology Regulated intramembrane proteolysis (RIP) pathways govern important processes in all three domains of life. A key component of RIP pathways is an intramembrane protease (IP), which cleaves one or more...

Inhibitory proteins of Bacillus subtilis interact within the membrane to block intramembrane protease activity

#JBacteriol from Lee Kroos

journals.asm.org/doi/10.1128/...

Ákos T Kovács

@evolvedbiofilm.bsky.social

6 months ago

Deciphering the function of Com_YlbF domain-containing proteins in Staphylococcus aureus | Journal of Bacteriology The Com_YlbF proteins are a group of small proteins identified in some Gram-positive bacteria whose function has not been described, but whose deletion has been related to pleiotropic effects in Bacillus subtilis (reduction of biofilm ability, sporulation,...

Deciphering the function of Com_YlbF domain-containing proteins in Staphylococcus aureus

in #JBacteriol

journals.asm.org/doi/10.1128/...

Ákos T Kovács

@evolvedbiofilm.bsky.social

8 months ago

Milestones in the development of Myxococcus xanthus as a model multicellular bacterium

#JBacteriol MiniReview by the Myxococcus field (about 15 labs)

journals.asm.org/doi/10.1128/...

Ákos T Kovács

@evolvedbiofilm.bsky.social

8 months ago

Distinct cell division features in Anabaena, a multicellular cyanobacterium | Journal of Bacteriology Cyanobacteria are oxygenic phototrophs that make an important contribution to the global primary productivity in the biosphere (1). Indeed, they are the organisms in which oxygenic photosynthesis, bas...

Distinct cell division features in Anabaena, a multicellular cyanobacterium

#JBacteriol Mini review by Antonia Herrero

journals.asm.org/doi/10.1128/...

Ákos T Kovács

@evolvedbiofilm.bsky.social

10 months ago

Azithromycin represses evolution of ceftazidime/avibactam resistance by translational repression of rpoS in Pseudomonas aeruginosa | Journal of Bacteriology Antibiotic resistance, a global public health challenge, demands the development of novel antibiotics and therapeutic strategies. Ceftazidime-avibactam (CZA) is a combination of a β-lactam antibiotic with a β-lactamase inhibitor that is effective against ...

Azithromycin represses evolution of ceftazidime/avibactam resistance by translational repression of rpoS in Pseudomonas aeruginosa

-in #JBacteriol

journals.asm.org/doi/10.1128/...

Ákos T Kovács

@evolvedbiofilm.bsky.social

10 months ago

FleQ finetunes the expression of a subset of BrlR-activated genes to enable antibiotic tolerance by Pseudomonas aeruginosa biofilms

#JBacteriol by Victoria Oladosu and @biofilms-karin.bsky.social

journals.asm.org/doi/10.1128/...

Ákos T Kovács

@evolvedbiofilm.bsky.social

10 months ago

The TerC family metal chaperone MeeY enables surfactin export in Bacillus subtilis

#JBacteriol from @johnhelmann.bsky.social
with Dan Kearns lab

journals.asm.org/doi/10.1128/...

Ákos T Kovács

@evolvedbiofilm.bsky.social

11 months ago

Colony morphotype variation in Burkholderia: implications for success of applications and therapeutics | Journal of Bacteriology The Burkholderia genus, comprising over 90 species (1, 2), is a member of the β-proteobacteria subphylum and is widely distributed in various environments such as the atmosphere, soil, water, plant rh...

Colony morphotype variation in Burkholderia: implications for success of applications and therapeutics

#JBacteriol by @pmlcoulon.bsky.social et al

journals.asm.org/doi/10.1128/...

Ákos T Kovács

@evolvedbiofilm.bsky.social

11 months ago

Bacillus subtilis MurJ and Amj Lipid II flippases are not essential for growth | Journal of Bacteriology The assembly of peptidoglycan (PG), the typically essential polymer that provides structural integrity to bacterial cells, begins with the synthesis of the Lipid II monomer in the cytoplasm and along ...

Bacillus subtilis MurJ and Amj Lipid II flippases are not essential for growth

#JBacteriol by Kiera Englehart & Jonathan Dworkin

journals.asm.org/doi/10.1128/...

Ákos T Kovács

@evolvedbiofilm.bsky.social

11 months ago

Controlled burn: interconnections between energy-spilling pathways and metabolic signaling in bacteria

#JBacteriol Mini Review by Nicolaus Jakowec and Steven Finkel

journals.asm.org/doi/10.1128/...

Ákos T Kovács

@evolvedbiofilm.bsky.social

1 year ago

Germination of Bacillus spores by LiCl | Journal of Bacteriology The ability of some bacteria to form spores upon nutrient starvation confers properties of metabolic dormancy and enhanced resistance to environmental stressors that would otherwise kill vegetative ce...

Germination of Bacillus spores by LiCl

-in #JBacteriol from Graham Christie, Peter Setlow

journals.asm.org/doi/10.1128/...

George O'Toole (he/him/his)

@geiselbiofilm.bsky.social

1 year ago

New in JB: Alaei, Jain et al. explore how changes to lipidA lipid A influences P. gingivalis OMV production and associated phenotypes. They also link OMV production to biofilm formation.
journals.asm.org/doi/10.1128/...
@asm.org. #JBacteriol

Ákos T Kovács

@evolvedbiofilm.bsky.social

1 year ago

A novel regulation on the developmental checkpoint protein Sda that controls sporulation and biofilm formation in Bacillus subtilis | Journal of Bacteriology Genome-wide ribosome profiling in Bacillus subtilis shows that under serine limitation, ribosomes pause on the four TCN (N for A, C, G, and T), but not AGC/AGT serine codons, during translation at a g...

A novel regulation on the developmental checkpoint protein Sda that controls sporulation and biofilm formation in Bacillus subtilis

#JBacteriol from Yunrong Chai

journals.asm.org/doi/10.1128/...

Ákos T Kovács

@evolvedbiofilm.bsky.social

1 year ago

Recent progress in proteins regulating the germination of Bacillus subtilis spores | Journal of Bacteriology Bacteria in orders of Bacillales and Clostridiales can undergo sporulation to form dormant and resistant spores for surviving adverse environments such as nutrient limitation (1). The process of sporu...

Recent progress in proteins regulating the germination of Bacillus subtilis spores

- in #JBacteriol

journals.asm.org/doi/10.1128/...