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#OpenCloning

Latest posts tagged with #OpenCloning on Bluesky

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Posts tagged #OpenCloning

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🧬 Just pushed new updates to #OpenCloning Assembler based on user feedback!

✍️ Now supports #GoldenBraid and Plant #MoClo

πŸš€ You can edit assembly product names, view a full syntax overview, and enjoy cleaner visuals.

πŸ‘‰ Try it out (link in comments) β€” feedback welcome!

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#OpenCloning is shaping up to be a really useful tool, and the new Assembler is powerful for defining and using Golden Gate syntaxes. Give it a try!

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Someone requested #GoldenBraid Assembly, now it's live on #OpenCloning. Still missing the omega Level 1 assembly (coming very soon).

For GoldenBraid reference: goldenbraidpro.com and pmc.ncbi.nlm.nih.gov/articles/PMC...

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Do you like #cloning or #MolBiol?
Do you like #OpenSource software?
Do you like keeping structured research records in a way that can also save you time and help avoid mistakes?

If you answered 'Yes' to any of the above, then check out this new update to #OpenCloning from @manu-lera.bsky.social πŸ‘‡

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πŸš€ Excited to launch the #OpenCloning Assembler!

🧬 Built to demystify Golden Gate for beginners and save time for experts -> MoClo assembly blazing fast ⚑

✍️ Use existing syntaxes or design your own!

#SynBio #GoldenGate #MoClo #OpenSource #Biotech

πŸ‘‰ Try it: app.opencloning.org

Feedback welcome!

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Working on a tool to define Golden Gate syntaxes for #OpenCloning. You define your syntax, and then you can drag-and-drop your plasmids in OpenCloning, they will be sorted into the right categories, and you can do combinatorial assembly. Reach out if you'd like to contribute:

syntax.opencloning.org

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πŸš€ A long-requested feature just landed in #OpenCloning

πŸ”¬πŸ§¬ You can now manually annotate sequences and create primers right from the editor

✨ Hope it makes your workflow a bit smoother!

πŸ“¨ If you want to stay updated with upcoming features, subscribe to the mailing list (link in the comments)

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If you are looking for an Open and free alternative to SnapGene, you may want to give #OpenCloning a try!

bsky.app/profile/manu...

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If you are looking for an Open and free alternative to SnapGene, you may want to give #OpenCloning a try!

bsky.app/profile/manu...

cc: crisprflydesign.bsky.social

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If you are looking for an Open and free alternative to SnapGene, with a more modern look than ApE, you may want to give #OpenCloning a try!

bsky.app/profile/manu...

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If you are looking for an Open and free alternative to SnapGene, you may want to give #OpenCloning a try!

bsky.app/profile/manu...

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If you are looking for an Open and Free alternative to SnapGene, you may want to give #OpenCloning a try!

bsky.app/profile/manu...

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A cloning strategy as depicted in the OpenCloning website

A cloning strategy as depicted in the OpenCloning website

#OpenCloning is a an Open Source alternative to SnapGene/Benchling that supports automation and integration with other software

βœ… Free
πŸ”“ Open Source
🧬 More cloning methods than SnapGene
πŸ€– Can be automated with python
πŸ‘¨β€πŸ”¬ Built by a researcher β€” for researchers!

πŸ‘‰ Check it out at opencloning.org

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✨ Last week at #ESSL2025 I presented a poster on #OpenCloning 🧬

πŸ”₯ Crowd favorite feature: pull gene sequences directly from NCBI β€” no copy-paste needed!

πŸŽ₯ Here’s a quick demo

πŸ”— Visit opencloning.org

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#OpenCloning opencloning.org now features WeKwikGene integrationβ€”streamlining downloading plasmid sequences from wekwikgene.wllsb.edu.cn and downstream cloning and collaborations!

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πŸš€ OpenCloning now has a documentation page!

Whether you're getting started or diving deep, find everything you need to make the most of OpenCloning, including a powerful search function to quickly find exactly what you’re looking for.

#OpenCloning #OpenSource #SynBio

πŸ”— docs.opencloning.org

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GoldenPiCS with OpenCloning: Step-by-Step MoClo Cloning Tutorial
GoldenPiCS with OpenCloning: Step-by-Step MoClo Cloning Tutorial YouTube video by Genestorian

πŸ”¬ I added a template for the #GoldenPiCS #MoClo kit to #OpenCloning!
⚑ Speed up your design when cloning in #Kphaffii!

πŸ§ͺ Simulate plasmid assembly
🧬 …and integration into the genome!

πŸ‘‰ Check it out here: opencloning.org 🌍
πŸŽ₯ youtu.be/BadWzLjojb4

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πŸ§ͺ Maybe it's time to give #OpenCloning a try!

βœ… Free
πŸ”“ Open Source
🧬 More cloning methods than SnapGene
πŸ‘¨β€πŸ”¬ Built by a fellow researcher β€” for researchers!

πŸ‘‰ Check it out: bsky.app/profile/manu...

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I'm really excited that #OpenCloning has been integrated into @elabftw.bsky.social - we're just getting it setup on our development server, and I'm looking forward to seeing what our users will be able to do with this! Love the structure of your approach - keep up the good work!

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London SynBio Network 8 London SynBio afterwork talks and networking event on Thursday 24th of April

🧬 If you are in London and you want to learn about a free & Open Source SnapGene/Benchling alternative that also supports automation πŸ€–, come to our next London Synbio Network meeting this Thursday 24th in Imperial!πŸŽ“

🎀 I’ll be giving a talk about #OpenCloning!

events.humanitix.com/copy-of-lond...

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Come join us for the next London Synbio Network meeting on Thursday the 24th of April in Imperial.

I am giving a talk about #OpenCloning, so join if you want to hear more about Open Source and automation of DNA engineering.

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Just got your sequencing results from Full Circle Labs? πŸ§¬βœ… Use #OpenCloning to easily align your Nanopore (or Sanger) data with your constructs and verify your sequences in seconds!

πŸ”¬ Free & Open Source
πŸ› οΈ Supports most cloning methods

πŸ”— opencloning.org

#SyntheticBiology #MolecularBiology

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#OpenCloning is an amazing tool, and we've integrated it into the next version of eLabFTW!

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You can now βœ… verify your #OpenCloning sequences using sequencing data 🧬 (Sanger or Nanopore). Just load your files πŸ“‚ and see where they align! 🎯

#OpenCloning is free & Open Source, and supports most cloning methods! πŸ”¬πŸ› οΈ

πŸ‘‰ Try it here: opencloning.org
🎞️ Demo video: youtu.be/n0hedzvpW88?...

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#OpenCloning now displays primer details! πŸ§¬πŸ” Minimal info is shown in the table, and the full #primer3 report when clicking the ℹ️ icon.

πŸ§ͺ The values in parenthesis are for the full primer, the preceding ones for the annealing part.

πŸ”— Try it yourself: opencloning.org
πŸŽ₯ Demo: youtu.be/-wjOY4i6mjg

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With #OpenCloning, you can very quickly access your locus of interest from the #NCBI to start cloning. No more downloading entire genomes and cutting your fragment of interest!

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Gateway primer design with ShareYourCloning
Gateway primer design with ShareYourCloning YouTube video by Genestorian

🧬 Using Gateway for cloning? Plan it with #OpenCloning! πŸ–₯️✨

πŸ†“ Free & Open Source, it supports most cloning methods! πŸ”¬πŸ› οΈ

πŸŽ₯ Demo: youtu.be/-wjOY4i6mjg
πŸ§ͺ Try it yourself: opencloning.org

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πŸ€” Why GateWayMine?

I'm building #OpenCloning, an 🧬 Open Source web app to plan & document cloning & genome engineering. πŸ–₯️✨

To support Gateway Cloning, I needed consensus sequences for att sitesβ€”but they were nowhere to be found! πŸ˜±πŸ”

⛏️ So, I had to mine them! ⛏️

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🧬 Now you can access @sevaplasmids.bsky.social directly from #OpenCloning ✨.

πŸ”— try it: opencloning.org
🎞️ demo: youtu.be/n0hedzvpW88?...

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I have turned the primer designer of #OpenCloning into a stepper after feedback from lab members and @francescodb.bsky.social. It now conveys more clearly the workflow. Below an example of #primerDesign for #GatewayCloning

πŸ”— try it: opencloning.org
🎞️ demo: www.youtube.com/watch?v=n0he...

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