Appropriate expression of exogenous Nr5a2 enhances SCNT efficiency. Immunofluorescence staining of blastocysts in each group (SCNT group and Nr5a2 overexpression groups; 100, 200, and 500 ng/μl). DAPI (blue) stains nuclei, SOX2 (green) marks the inner cell mass, and CDX2 (red) labels the trophectoderm. Scale bar = 100 µm.
Somatic Cell Nuclear Transfer (SCNT) enables generation of an #embryo from a somatic cell, but #epigenetic barriers limit its efficiency. This study shows that Nr5a2 overexpression enhances #SCNT embryo development by improving genome activation & recruiting P300 @plosbiology.org 🧪 plos.io/4bl8htJ