![Smc3 protein depletion protects lateral line neuromasts from neomycin cytotoxicity. (A) Representative images of lateral line neuromasts in SC-KD larvae and Smc3-KD larvae at 5 dpf with and without neomycin treatment and stained with 2-[4-(Dimethylamino)styryl]-1-ethylpyridinium iodide (DASPEI). Scale bars: 100 µm. (B) Quantification of neuromast survival after neomycin treatment for each injection type. Neuromast survival was calculated as the ratio of observed neuromasts in each neomycin-treated embryo to the average number of neuromasts visible in control-treated embryos of the same injection type (e.g. SC-KD or Smc3-KD). By this method, ratios greater than 1 may occur if the number of observed neuromasts in a neomycin-treated embryo exceeds the average number of neuromasts observed in the relevant control-treated embryos. Student's t-test, ****P<0.0001, SC-KD n=40, Smc3-KD n=36.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:ckg56sce76vdnqq4yhdlfbts/bafkreibxojvnwje4ncfe2zn3hv5eihsy2w5pirtiv6p6bsyulqd322wzwe)
Smc3 protein depletion protects lateral line neuromasts from neomycin cytotoxicity. (A) Representative images of lateral line neuromasts in SC-KD larvae and Smc3-KD larvae at 5 dpf with and without neomycin treatment and stained with 2-[4-(Dimethylamino)styryl]-1-ethylpyridinium iodide (DASPEI). Scale bars: 100 µm. (B) Quantification of neuromast survival after neomycin treatment for each injection type. Neuromast survival was calculated as the ratio of observed neuromasts in each neomycin-treated embryo to the average number of neuromasts visible in control-treated embryos of the same injection type (e.g. SC-KD or Smc3-KD). By this method, ratios greater than 1 may occur if the number of observed neuromasts in a neomycin-treated embryo exceeds the average number of neuromasts observed in the relevant control-treated embryos. Student's t-test, ****P<0.0001, SC-KD n=40, Smc3-KD n=36.
Fiona Mensching & co show the first functional data showing that Smc3 contributes to hair cell activities. doi.org/10.1242/bio....
#Smc3 #Hair cells #Zebrafish #Otoliths
