Figure 5 | Application of the photobleacher to the pigment-rich volumetric tissues after clearing. (A) Schematic illustration of the photobleaching process. The tissues were illuminated after clearing, with hydrogen peroxide included when necessary. (B) Mouse hearts with various photobleaching conditions. Representative single z-planes are shown. (C) Three-dimensional rendering of a mouse heart after IHC staining using an anti-αSMA antibody. (D) Pieces of human cortex from an identical donor, with various photobleaching conditions. Three-dimensional renderings are shown. Outlines of tissues were shown in dot lines when necessary. (E) Three-dimensional rendering of a human cerebral cortex after IHC staining using anti-αSMA and anti-GFAP antibodies.
Here's an interesting way to do photobleaching with cleared tissue: Add 0.1% H2O2 to the clearing medium and blast with LEDs for 24h at RT. Also includes very detailed instructions for building an LED photobleaching setup - the samples ate placed between two […]
[Original post on mstdn.science]