Latest posts tagged with #Transgene on Bluesky
Ground breaking 2026 article by Nagalakshmi et al.:
‘High-efficiency, transgene-free plant genome editing by viral delivery of an engineered TnpB’
tinyurl.com/bdfndr7s
#WIS
#CRISPR
#Transgene
#GeneEditing
#RNA
#Rybozyme
#SelfCleaving
#Bleach
#Phenotype
Extended data fig.8
wisdeppes.bsky.social
@wisdeppes.bsky.social
· 2m
Ground breaking 2026 article by Nagalakshmi et al.:
‘High-efficiency, transgene-free plant genome editing by viral delivery of an engineered TnpB’
tinyurl.com/bdfndr7s
#WIS
#CRISPR
#Transgene
#GeneEditing
#RNA
Extended data fig.5
Ground breaking 2026 article by Nagalakshmi et al.:
‘High-efficiency, transgene-free plant genome editing by viral delivery of an engineered TnpB’
tinyurl.com/bdfndr7s
#WIS
#CRISPR
#Transgene
#GeneEditing
#RNA
#Rybozyme
#SelfCleaving
#Bleach
Extended data fig.5 caption
Ground breaking 2026 article by Nagalakshmi et al.:
‘High-efficiency, transgene-free plant genome editing by viral delivery of an engineered TnpB’
tinyurl.com/bdfndr7s
#WIS
#CRISPR
#Transgene
#GeneEditing
#RNA
#Rybozyme
#SelfCleaving
#Bleach
Extended data fig.4
Ground breaking 2026 article by Nagalakshmi et al.:
‘High-efficiency, transgene-free plant genome editing by viral delivery of an engineered TnpB’
tinyurl.com/bdfndr7s
#WIS
#CRISPR
#Transgene
#GeneEditing
#RNA
#Rybozyme
#SelfCleaving
#Bleach
Extended data fig.4 caption
Ground breaking 2026 article by Nagalakshmi et al.:
‘High-efficiency, transgene-free plant genome editing by viral delivery of an engineered TnpB’
tinyurl.com/bdfndr7s
#WIS
#Transgene
Fig. 1 Virus delivery of the enhanced eTnpBc variant results in efficient systemic somatic editing
Fig.1g
- a hand-in-hand collaboration with K.RITTNER & E.Quéméneur #Transgene , 🦠
- spearheaded by @hyennev.bsky.social in our team 👨💻 ,
- and executed by #L.WALTHER (soon defending) 👨🔬 .
@inserm.fr @unistra.fr #CRBS
Fig. 2 Effect of transgene fusion on native expression. (a) Selection of natively high expressed genes (Schmollinger et al., 2014). (b) Schematic of a highly expressed gene locus. Repair template integrated in native coding sequence and is composed of 50-homology arm, the FMDV 2A peptide, mVenus and aadA fusion CDS and 30-homology arm. (c) Relative mRNA fold change of respective gene for biological triplicates compared with UVM4. Error bars (SD) were calculated from technical triplicates. PCR products were separated in agarose gels (Fig. S5). (d) Western blot with immunodetection (anti-GFP for YFP-aadA fusion protein) and Coomassie Brilliant Blue (CBB) as loading control. (e) Single-cell microscopy of Chlamydomonas reinhardtii showing yellow fluorescent protein (YFP) emission, Chl emission, a combined overlay as well as a differential interference contrast (DIC) picture for UVM4, cytosolic and chloroplast control (PSAD), representative RPL10, RBCS2 and Light harvesting Chl a/b binding protein of LHCII (LHCBM1) transformants.
Fig. 4 Biotechnological approach. (a) Reaction from Farnesylpyrophosphate (FPP; originating from methylerythritol phosphate pathway) to valencene through the Callitropsis nootkatensis Valencene synthase. (b) Schematic constructs for random insertion and scar-less targeted integration of the CnVs in between native coding sequence and 30-UTR. (c) Valencene production by Chlamydomonas reinhardtii is presented for best random integrated (n = 20) and scar-less integrated CnVs after Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit 2 (RBCS2) (n = 3) and Light harvesting Chl a/b binding protein of LHCII (LHCBM1) native loci (n = 6). For random integrated CnVs, best 20 expressing transformants were chosen based on yellow fluorescent protein (YFP) fluorescence out of 288 random isolated transformants. displays mean production of each group and error bars represent the SD. Asterisks (*) indicate the significance level of an unpaired, two-tail Students t-test for mean production assuming unequal variances (**, P < 0.01).
Great work by Jacobebbinghaus et al. (2025) on transcriptional gene fusions via #CRISPR/Cas9-mediated targeted integration at safe harbors (e.g. LHCBM1 locus, 50 bp homology arms) to enhance #transgene expression in #Chlamydomonas compared with randomly-inserted transgenes.
🔗 doi.org/10.1111/nph....
The image shows a false-colored confocal microscopy image of the locus coeruleus of a Th-cre mouse. LC-NE neurons have been visualized by an antibody staining against tyrosine hydroxylase (cyan), while cre mediated expression of a reporter protein is visible around the LC (red). Image Credit: Lena S. Eschholz, Alexander Dieter, Chantal Wissing.
This study reveals substantial heterogeneity in #transgene expression patterns when using different strategies to virally transduce #NoradrenergicNeurons of the #LocusCoeruleus, highlighting the need for caution to avoid misinterpretations when studying LC function @plosbiology.org 🧪 plos.io/3IehTKb
New study online today! We present a method to identify insertion sites of Tol2 transgenes in zebrafish #zebrafish #Tol2 #Transgene www.cell.com/cell-reports...
The image shows a false-colored confocal microscopy image of the locus coeruleus of a Th-cre mouse. LC-NE neurons have been visualized by an antibody staining against tyrosine hydroxylase (cyan), while cre mediated expression of a reporter protein is visible around the LC (red). Image Credit: Lena S. Eschholz, Alexander Dieter, Chantal Wissing.
This study reveals substantial heterogeneity in #transgene expression patterns when using different strategies to virally transduce #NoradrenergicNeurons of the #LocusCoeruleus, highlighting the need for caution to avoid misinterpretations when studying LC function @plosbiology.org 🧪 plos.io/3IehTKb
The image shows a false-colored confocal microscopy image of the locus coeruleus of a Th-cre mouse. LC-NE neurons have been visualized by an antibody staining against tyrosine hydroxylase (cyan), while cre mediated expression of a reporter protein is visible around the LC (red). Image Credit: Lena S. Eschholz, Alexander Dieter, Chantal Wissing.
This study reveals substantial heterogeneity in #transgene expression patterns when using different strategies to virally transduce #NoradrenergicNeurons of the #LocusCoeruleus, highlighting the need for caution to avoid misinterpretations when studying LC function @plosbiology.org 🧪 plos.io/3IehTKb
New #OpenAccess work in #RESMedVetEnt now available!
Extended time to maturity in #Anopheles coluzzii: Implications of late egg hatch for vector control and #transgene fitness
doi.org/10.1111/mve.12814
#InsectVectors #VectorControl
@wiley.com
