Latest posts tagged with #UExM on Bluesky
At this high resolution, we also caught something new: Using the new #HAK-Actin #UExM probe, we saw that
#Actin accumulates at the furrow base before compartments fully seal ➡️ they're still open at the bottom.
➡️ The #multicellular epithelium-like stage is shorter than we previously proposed.
Using #UExM, we could finally see the MT network. Two populations:
Cortical MTs=from MTOCs to the cortex, spacing nuclei evenly
Furrow-tracking MTs=elongating with the ingressing membrane.
Thx for @mollyherring.bsky.social for featuring our recent work with @centriolelab.bsky.social and our quest with @gautamdey.bsky.social & the support of the @moorefound.bsky.social to look for the beauty in every #eukaryote out there using #Expansion #Microscopy #UExM
Tour de force #UExM study of #protist #cytoskeleton structures (>200 species🤯), led by @dudinlab.bsky.social, @gautamdey.bsky.social. A must for #tubulin & #centrin aficionados🤩
www.cell.com/cell/fulltex...
Top row images showing cells of the tintinnid ciliate Schmidingerella from early to late division stages. Shell-forming material ist stained in purple/red. Bottom row images show 3D models of the material for volumetric analyses.
We recently investigated how much and when tintinnid ciliates produce shell material during the cell cycle to construct a new shell (lorica) after division: tinyurl.com/49usp8xw
We adapted a classical staining technique.
Still no #UExM for tintinnids dudinlab.bsky.social? (wink)
#protistsonsky
#Microbial #Eukaryotes in #UExM on the cover of @natgenet.nature.com ! Unbelievable !!!
Also read the amazing work by @alexdemendoza.bsky.social and co !!!
🥳 Our new paper on C2CD3 is out in @plosbiology.org
With the labs of @cellarchlab.com , @chgenoud.bsky.social , and @stearnslab.bsky.social , we revealed the mammalian centriole Distal Ring architecture by combining #UExM and in situ #CryoET.
Big congrats to @ebertiaux.bsky.social and all involved.
Happy to share our fun team-up with @centriolelab.bsky.social & @stearnslab.bsky.social to reveal the architecture of the mammalian centriole’s Distal Ring 🛟. Very cool multiscale integration of #UExM 📏🔬with #CryoET ❄️🔬. Congrats to @ebertiaux.bsky.social & @computingcaitie.bsky.social #TeamTomo 🧪
Team led by Hiroki Shibuya revealed major centriolar structural changes during flagellar assembly of mouse #sperm using ultrastructure expansion microscopy #UExM and identified Poc5 protein as a key factor ensuring structural integrity of sperm flagellum.
In #ScienceAdvances
doi.org/10.1126/scia...
So i'm usually interested about how cells divide in the cold, but couldnt resist helping out a tiny bit for a Euk dividing at 63°C.
Congrats to the whole team @hbrappap.bsky.social & Kudos to @fmikus.bsky.social for yet again the almighty #UExM !
Next up @computingcaitie.bsky.social from @cellarchlab.com marvels us with in situ structural biology of mammalian motile cilia base- power of #cryoET with #XL-MS & #UExM! Stunning cataloguing of MIPs in different axial positions & cilia types, filamentous actin in action. #UKCilia2025 /3
#uexm of human airway motile cilia
I mean, how does Microtubules & centrin cytoskeleton shapes the suctorian resting and feeding tip ? Just how ?
#UExM #Expansion #Microscopy
@zjmaggiexu.bsky.social sent samples,fixed differently to see which can allow #UExM & Marine, our Lab Manager Extraordinaire,powered through. Its not the easiest sample, some protective layer hinders homogenous ExM of the cell body all the time,but the reaction we got when it worked was worth it n/2
In French - Virginie on Swiss radio! 🎙️ She talks about our latest collab with @dudinlab.bsky.social and @gautamdey.bsky.social #UExM #ProtistsOnSky
Exciting news: the new review from @centriolelab.bsky.social is out in the amazing #JCSciliaSI! 🎉
I had the pleasure of working alongside Olivier Mercey, Paul Guichard and Virginie Hamel on this one.
Cherry on top: our #UExM image was selected for the cover of the issue! 🤩
We’re not leaving our beloved #Ichthyosporeans ❤️ / they remain core. But this marks our first big step beyond them, using #UExM to explore how #multicellularity evolved across lineages; and using #Microscopy to see in 3D how cells organize, divide & come together. 🧫🌍
You know it will be #UExM
An absolute diamond of cell biology work. Congratulations 🎉 to all authors #UExM #Parasitesrule #CellBiology #Cilia
🧬 New tool for U-ExM!
HAK-Actin is now available from Spirochrome → a reliable probe to label actin filaments in expansion microscopy.
Check it out at Spirochrome!
#UExM #Actin #Cytoskeleton #SuperResolution
A new #UExM probe for #Actin!
Fun times thinking/developing/testing with @centriolelab.bsky.social & @lreymond.bsky.social !
Congrats to all !
If interested in #UExM, or #HAK-Actin, feel free to reach out !
Soon available at @spirochrome.com
#Expansion #Microscopy #ProtistsOnSky
Congrats to the whole team.
It was quite fun to teach @kristina-prokina.bsky.social how to #UExM
If i'm not mistaken, this would be the first intance of new species discription using some #UExM.
I know it won't be the last !
The list of successfully fixed species keeps getting longer and longer. Can’t wait for the first #UExM images fresh out of our new scopes…
Amazing job @fmikus.bsky.social and thanks @pjkeelinglab.bsky.social, @fburki.bsky.social, Denis, Vittorio and all for joining forces !
There was even some time to expand with many labmembers - although some cells were incredibly hard to find! Thanks @nicolecoots.bsky.social for spending hours looking for them and @ambonacolta.bsky.social for providing large samples that could not be missed!
#UExM #expandeverything #protistsonsky
Happy to have our #UExM students for the next two weeks!
Rachid & Sneha coming directly from NYU #AbuDhabi& Andrea from Innsbruck University
More #Microbial #Eukaryotes! also some Fish tissues!
JOIN US with @gautamdey.bsky.social @fmikus.bsky.social on this @moorefound.bsky.social funded adventure.
I'll be around a bit from Tomorrow at #ICOP2025
If anyone wants to chat, grab a coffee or talk about
#Ichthyosporea, #Expansion #Microscopy #UExM
#ProtistsOnSky #Protists
Woo! Very excited to share our collaborative work on the centriole's Luminal Distal Ring ⭕. This project started 13 years ago with @hugovdhoek.bsky.social & @stearnslab.bsky.social, and then beautifully came together with @computingcaitie.bsky.social & @centriolelab.bsky.social.
#TeamTomo #UExM 🧪🧶🧬🔬
🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
Expansion microscopy image, and accompanying sketch, of S. arctica nucleus with labelled microtubules and nuclear pore complexes.
9/ The observed patterns matched our model, and their parameters place these systems near the predicted optimal filtering regime -- These NPCs may act as efficient spatial thresholding filters! #Microscopy #QuantBio #Microtubules #UExM
