#CellSky

Daniel MacPhee 🇨🇦

@d-macphee.bsky.social

1 month ago

Aging researchers find new puzzle piece in the game of longevity The lab of Kris Burkewitz just made a key discovery: How cellular machineries are structured and organized within a cell has implications for healthy aging. “We didn't just add a piece to the puzzle—w...

The original post from Vanderbilt
#CellSky #Aging #CellBio 🧪
medschool.vanderbilt.edu/basic-scienc...

Daniel MacPhee 🇨🇦

@d-macphee.bsky.social

1 month ago

A hidden cellular process may drive aging and disease As we age, our cells don’t just wear down—they reorganize. Researchers found that cells actively remodel a key structure called the endoplasmic reticulum, reducing protein-producing regions while pres...

“research shows that cells actively reshape the endoplasmic reticulum or ER…Rather than remaining static the ER undergoes controlled remodeling as organisms grow older. The team discovered that this remodeling happens through a process called ER-phagy”
🧪 #CellSky
www.sciencedaily.com/releases/202...

Victor Tatarskiy

@greenrat.bsky.social

5 months ago

On the left - western blot of B16F10 cells wt and KO for CDK8. Our in house produced antibodies give a lot of unspecific bands. On the right same probes with antibodies preincubated with fixed CDK8 KO cells - there is a specific band and faint unspecific bands, which can be probably eliminated with increase of amount of KO cells.

Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky

Hannah Reich

@hannahgreich.bsky.social

1 year ago

Classes start in a week (ah!) so re-sharing this!

Still soliciting suggestions for emerging model organisms for cell bio. "Model" can be interpreted broadly but looking for taxa that:

1) have an active research community
2) some combo of a genome, life cycle, culture.

#CellSky #AcademicSky