#DiabetesScience

Innovative Science Accelerator Program

@www.isac-kuh.org

3 months ago

Genetics play a role — not everyone with similar blood sugar control develops the same diabetes complications. Genetics may help explain why some people avoid complications even with high HbA1c. 🧬 #DiabetesScience #BeyondType1

Diabetologia

@diabetologiajnl.bsky.social

5 months ago

3D electron microscopy reveals novel ultrastructural changes in the diabetic retinal neurovascular unit - Diabetologia Aims/hypothesis This study used serial block-face scanning electron microscopy (SBF-SEM), a nanoscale imaging technique in x-y-z planes, to investigate 3D ultrastructural changes in the retinal neurovascular unit (NVU) associated with diabetes. We hypothesised that this approach would reveal previously uncharacterised pathological alterations that contribute to the development of diabetic retinal disease (DRD). Methods Retinas from male diabetic and non-diabetic mice, as well as from human male donors with and without diabetes, were prepared for SBF-SEM imaging. Retinal tissue was microdissected, fixed and embedded for serial sectioning and 3D reconstruction. Ultrastructural analysis of the NVU was performed in capillary regions exclusively within the superficial vascular plexus of both mouse and human retinas. Image stacks were processed using Microscopy Image Browser for contrast normalisation and segmentation, with 3D visualisation performed in Amira software. Quantitative analyses were conducted on pericyte–endothelial cell peg-and-socket formations, cell–basement membrane (BM) interactions, endothelial tubule formation and vascular BM thickness. Results SBF-SEM revealed novel 3D ultrastructural changes in the retinal NVU of diabetic mice and humans, including: (1) partial detachment and reduced frequency of pericyte–endothelium peg-and-socket formations (p<0.05–0.001); (2) localised detachment of endothelial cells and pericytes from the vascular BM (p<0.05–0.01), along with macroglial cell retraction from the outer vascular BM; and (3) increased formation of endothelial tubules (p<0.01–0.001). These changes were observed in the absence of any obvious vascular BM thickening, as no significant differences in mean or maximum BM thickness were found between diabetic and non-diabetic retinal capillaries analysed in this study. Conclusions/interpretation This study provides new insights into the early ultrastructural changes in the retinal NVU in DRD, offering a basis for a better understanding of the pathological processes that contribute to the development of this disease. Data availability Links to all raw image stacks analysed in this article are available at https://doi.org/10.5281/zenodo.15210333 . The MATLAB vascular BM thickness measurement script is available at the GitHub link https://github.com/Curtis-WWIEM/BM_thickness . Graphical Abstract

Serial block-face SEM shows novel 3D ultrastructural changes in the retinal neurovascular unit in diabetic retinal disease. #DiabeticRetinopathy #VisionResearch #NeurovascularUnit #DiabetesScience #3DElectronMicroscopy #SBFSEM link.springer.com/article/10.1... 🔓

Diabetologia

@diabetologiajnl.bsky.social

6 months ago

Scavenging acrolein with 2-HDP preserves neurovascular integrity in a rat model of diabetic retinal disease - Diabetologia Aims/hypothesis Diabetic retinal disease (DRD) is characterised by progressive neurovascular unit (NVU) dysfunction, often occurring before visible microvascular damage. Our previous studies suggested...

#2-HDP protects against diabetic retinal neurovascular dysfunction in the diabetic rat retina, independent of glycaemic control. #DiabeticRetinopathy #VisionResearch #NeurovascularUnit #DiabetesScience #DrugDiscovery link.springer.com/article/10.1... 🔓

marc hall

@paulhall.mastodon.social.ap.brid.gy

6 months ago

🔬 20 years ago: Finger pricks & guessing
2024: 3-year implantable sensors coming soon
⚡ Over-the-counter CGMs for non-diabetics
🧠 AI nutrition recommendations
The blood sugar revolution is HERE
healthybloodsugarlevels.online/blood-sugar-studies

#BloodSugarStudies
#DiabetesResearch […]

Monica Westley PhD

@westerly2easterly.bsky.social

1 year ago

Welcome! You are invited to join a meeting: the(sugar)science presents Indiana SOM Site Visit: Diabetes Moonshots: Changing the Course of Type 1 Diabetes. After registering, you will receive a confirm... Biomarkers, Screening and the Role of IU in discovering the pathology of Type 1 Diabetes. *plus- a sneak peak at IU's exciting new facilities! Panelists: Carmella Evans-Molina MD PhD Emily K Sims MD...

🚨 Tomorrow’s the day! Join the(sugar)science at 10 AM ET for an exclusive Site Visit at IU SOM: Diabetes Moonshots.

RSVP now and be part of the innovation: us02web.zoom.us/meeting/regi...

#T1D #DiabetesScience #ResearchForward

Monica Westley PhD

@westerly2easterly.bsky.social

1 year ago

🔬 Th1nk Tank: Beta Cell Dysfunction During T1D Pathogenesis 🧬

Join us to explore insights from live pancreas tissue slices and their role in understanding beta cell function and immune activity in T1D!

🌐 Register here: us02web.zoom.us/meeting/regi...

#T1DResearch #BetaCellBiology #DiabetesScience