#DirectRNAseq

Javier Santoyo

@jsantoyo.bsky.social

3 months ago

RMPore: a comprehensive database of single-molecule RNA modifications detected by Nanopore direct RNA sequencing. #RNAmodifications #Nanopore #DirectRNAseq #Bioinformatics #Genomics #NAR @narjournal.bsky.social 🧪🧬 🖥️
academic.oup.com/nar/advance-...

Javier Santoyo

@jsantoyo.bsky.social

4 months ago

Establishing benchmarks for quantitative mapping of m6A by Nanopore Direct RNA Sequencing. #DirectRNAseq #RNAsequencing #RNAmodifications #m6A #Genomics #Bioinformatics @nanoporetech.com 🧪🧬 🖥️
www.biorxiv.org/content/10.1...

Javier Santoyo

@jsantoyo.bsky.social

4 months ago

Establishing benchmarks for quantitative mapping of m6A by Nanopore Direct RNA Sequencing. #DirectRNAseq #Sequencing #RNAmodifications @nanoporetech.com
www.biorxiv.org/content/10.1...

Javier Santoyo

@jsantoyo.bsky.social

4 months ago

DirectRM: integrated detection of landscape and crosstalk between multiple RNA modifications using direct RNA sequencing. #DirectRNAseq #RNAmodifications @nanoporetech.com #Sequencing @natcomms.nature.com 🧪🧬 🖥️
www.nature.com/articles/s41...

Javier Santoyo

@jsantoyo.bsky.social

9 months ago

Single-molecule direct RNA sequencing reveals the shaping of epitranscriptome across multiple species - Nature Communications Nanopore direct RNA sequencing is promising for epitranscriptome study but remains limited in single-molecule resolution. Here, the authors develop SingleMod, a tool for accurate single-molecule m6A…

Single-molecule direct RNA sequencing reveals the shaping of epitranscriptome across multiple species. #DirectRNAseq #Epitranscriptomics #SingleMoleculeSequencing #NanoporeSequencing
@nanoporetech.com‬ @natcomms.nature.com‬
www.nature.com/articles/s41...

Martin Smith

@martinalexsmith.bsky.social

8 years ago

FYI: BWA does not like uracil! Don't forget to "sed 's/U/T/g' rna.fastq" before aligning #directRNAseq data from #nanopore