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Learn more about adaptive sampling in our latest #Nanopore Know-How: nanoporetech.com/blog/guided-by-data-adap... #ACMGtg26

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Proud that this work is now published!

We hope it helps orient future #Nanopore long-read sequencing studies hunting for structural variants

Also really happy to share eight new high-coverage (>100x) long-read read pools and assemblies with the #Drosophila community!

doi.org/10.1093/g3jo...

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Last call for these 2 different #PhD positions in #nanopore tech:
πŸ—“οΈ apply by March 15 to join our team at the nano-dynamics group at @unibas.ch Switzerland.
Follow the guidelines online: schmid.chemie.unibas.ch/en/join-us-1/

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Mitotic microhomology-mediated break-induced replication promotes chromoanasynthesis - Nature Communications Chromoanasynthesis is an elusive form of complex chromosomal rearrangement commonly detected in cancer and congenital disorder. Here, the authors develop a long-read DNA sequencing approach and show t...

Excited to share our new paper in @NatureComms!
Using #nanopore sequencing, we uncover how mitotic microhomology-mediated break-induced replication #MMBIR can generate #chromoanasynthesis / complex genome rearrangements linked to cancer and congenital disorders.
www.nature.com/articles/s41...

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Hannah

Hannah

We are delighted to welcome Hannah to our group. She will conduct long-read sequencing to explore plant genomes. We are excited to have her on board and look forward to amazing discoveries ahead!

www.izmb.uni-bonn.de/en/pbb/team

#PlantGenomics #LongReadSequencing #Bioinformatics #Nanopore

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"We found that the elevated read counts and sequencing depth generated by Illumina NGS were offset by the greater read length obtained on the MinION MK1C and resulted in comparable pathogen genome coverage between the two platforms."

#Nanopore #DiscontinuedSequencers

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π˜Όπ™— π™žπ™£π™žπ™©π™žπ™€ π™™π™šπ™©π™šπ™˜π™©π™žπ™€π™£ 𝙀𝙛 𝙒π™ͺπ™‘π™©π™žπ™₯π™‘π™š π™šπ™₯π™žπ™©π™§π™–π™£π™¨π™˜π™§π™žπ™₯π™©π™€π™’π™žπ™˜ π™’π™€π™™π™žπ™›π™žπ™˜π™–π™©π™žπ™€π™£π™¨ 𝙛𝙧𝙀𝙒 π™Šπ™‰π™ π™™π™žπ™§π™šπ™˜π™© π™π™‰π˜Ό π™¨π™šπ™¦π™ͺπ™šπ™£π™˜π™žπ™£π™œ 𝙙𝙖𝙩𝙖

πŸ”— pubmed.ncbi.nlm.nih.gov/41603648/

#Nanopore #Bioinformatics
@nanoporetech.com

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New #OpenAccess research in #RESMedVetEnt

A multiplex #assay to detect #mosquito species, bloodmeal host source and #Plasmodium in #malaria vectors using #Nanopore amplicon #sequencing
doi.org/10.1111/mve.70055

#EntoMethods #InsectVectors #InsectBorneDiseases
@wileyecology.bsky.social

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Preview
Detection of DNA base modification using nanopore sequencing 2026 In this comprehensive two-day course, participants will learn how to use nanopore sequencing to detect DNA base modifications.

βœ…οΈ Registration is open for our Detection of DNA base modification using #nanopore #sequencing. We'll be covering experimental design, considerations for ultra-#longreads, visualisation of data, and comparison of approaches. πŸ§¬πŸ’»

πŸ“ Online (via Zoom)
πŸ“… Register by: 10 April

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🧬 #NARGAB New Research Spotlight
Direct #RNA #nanopore sequencing reveals the most detailed #m6A map to date across Human immunodeficiency virus 1 transcripts #HIV-1. @cnrs.fr @i2bcparissaclay.bsky.social
πŸ‘‰ Read the full paper: doi.org/10.1093/narg...

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But at the moment, @nanoporetech.com needs to make new clair3 models in pytorch for #SUP version 5.2.0 with move table support #nanopore

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www.biorxiv.org/content/10.6... Clair3 v2 for #nanopore reads, it keeps getting better!

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Anyone has tips to better balance the reads between barcodes with the @nanoporetech.com rapid barcoding kits? With the exception of 2 all these samples had an input of +/-200ng. There could ofc be small quantification/pippete errors but the difference between samples are sometimes huge. #nanopore

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A new NAR Genomics and Bioinformatics resource introduces SquiDBase, a community repository of raw #nanopore #microbiome sequencing data, supporting open access and broader utility for bioinformatics and comparative analyses. #Genomics
πŸ“„ https://doi.org/10.1093/nargab/lqaf213
πŸ‘€ EVBC: Daan Jansen

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Video

Learn more about adaptive sampling in our latest #Nanopore Know-How. Learn more here: nanoporetech.com/blog/guided-by-data-adap...

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Preview
Defining expansions and perturbations to the RNA polymerase III transcriptome and epitranscriptome by modified direct RNA nanopore sequencing - Nature Communications RNA polymerase III transcribes essential non-coding RNAs, but many aspects of this biology remain unclear. Here, the authors develop DRAP3R, a nanopore sequencing method that captures Pol III transcripts and RNA modifications, revealing new RNAs and dynamic modification patterns.

New paper uses a modified direct #RNA #nanopore sequencing framework (DRAP3R) to reveal expanded RNA polymerase III #transcriptome and epitranscriptome landscapes, including novel Pol III-transcribed RNAs. 🧬
πŸ“„ https://doi.org/10.1038/s41467-025-68230-1
πŸ‘€ EVBC member: Daniel Depledge

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Forgot to tag #nanopore if anyone has that tag in his/her feeds

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Original post on mastodon.green

Does anyone know if an NVidia RTX 4060 can handle an Mk1D MinION with runtime basecalling? ONT's documentation says that the minimum requirement is an RTX 5060, but this sounds a bit crazy. And the difference between the two GPUs is not that big I think.

Also, this will mostly be for dRNA-seq […]

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#PhDPosition #RibosomeBiology #TranslationQC #Biochemistry #Nanopore #YeastGenetics #JGU #Mainz #TRR319 #YouRMaP #ScienceJobs #Germany #BlueskyJobs

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"Libraries were sequenced on a PromethION P2 Solo device using FLO-PRO114M flow cells."

#Nanopore #DiscontinuedSequencers

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Image of "Sequencing platforms" (from Figure 1 of the paper): Flongle, MinION [Mk1B], PromethION2 solo [sic]

Image of "Sequencing platforms" (from Figure 1 of the paper): Flongle, MinION [Mk1B], PromethION2 solo [sic]

#Nanopore #DiscontinuedSequencers

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"Libraries were loaded onto two FLOPRO004RA flow cells and sequenced using the ONT PromethION 2 Solo platform, which resulted in 5.5 million and 8.4 million raw reads for the control and NFNB-infected samples with estimated N50 values of 1.21 kb and 0.98 kb"

#Nanopore #DiscontinuedSequencers

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"Using Oxford Nanopore Technologies SQK-LSK110 kit, 200 fmol of each library were loaded on different R9.4.1 flowcells and run separately on MinION MK1C."

#Nanopore #DiscontinuedSequencers

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Link to preprint: www.biorxiv.org/content/10.6...

We’d love your thoughts!

#longread
@pacbio.bsky.social
@nanoporetech.com

#metagenomics #pacbio #nanopore 12/

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Unlocking the full potential of nanopore sequencing: tips, tricks, and advanced data analysis techniques Abstract. Nucleic acid sequencing is the process of identifying the sequence of DNA or RNA, with DNA used for genomes and RNA for transcriptomes. Decipheri

From @narjournal.bsky.social #NARCriticalReviewsAndPerspectives | Unlocking the full potential of #nanopore sequencing: tips, tricks, and advanced data analysis techniques | #Bioinformatics #Genomics #OpenScience #NGS #OxfordNanopore 🧬 πŸ–₯️πŸ§ͺπŸ”“
⬇️
academic.oup.com/nar/article/...

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Given the recent #Nipah outbreak in India, here are two #Nanopore protocols for NiV genomic sequencing in support of rapid outbreak response:

IPC: www.protocols.io/view/sequenc...
icddr,b/CDC: journals.asm.org/doi/10.1128/...

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Yeast elongation factor homolog New1 protects a subset of mRNAs from degradation by no-go decay Abstract. New1 is a homologue of the essential yeast translation elongation factor eEF3. Lack of New1 has been shown to induce ribosome queuing upstream of

4 - Still lots to learn about this mysterious factor.
πŸ”— Read the paper: academic.oup.com/nar/article/...
Funded by @dfg.de via SFB TRR319-RMaP πŸ™
#RibosomeBiology #TranslationQC #RNA #Nanopore #Yeast #PhDPosition #DFG #RMaP #New1

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Introducing ViMOP, a user friendly #nanopore sequencing pipeline for untargeted #VirusGenome assembly that works with clinical and field data to deliver high quality consensus genomes with minimal setup.πŸ¦ πŸ”¬ #Bioinformatics
πŸ“„ https://doi.org/10.1093/bioinformatics/btaf687
πŸ‘€ EVBC member: Philippe Lemey

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"sequenced on a MinION Mk1C device with a FLO-MIN 114 R10.4.1 flow cell"

#Nanopore #DiscontinuedSequencers

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We can't wait to see you in Valencia this summer at VALT! Check out our exciting Line up of speakers below. Interested in sharing your work on #LongReadTranscriptomics ? Abstract submission deadline πŸ“… March 31st.
#LongTREC #LongReadSequencing #Nanopore #Pacbio

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