Latest posts tagged with #Endoderm on Bluesky
ᯓ➤The study shows how opposing morphogen #gradients can generate spatially organized human #endoderm in vitro, with distinct #anterior and #domains 🔄 formed within a single culture, using a microfluidic gradient approach based on the ibidi µ-Slide #Chemotaxis.
#development
In 1817, Heinz Christian Pander discovered that animal embryos develop three layers: ectoderm, mesoderm, and endoderm. #Poetry #Science #History #Embryology #Pander #Ectoderm #Mesoderm #Endoderm sharpgiving.com/Sharp/theboo...
💡Application Note: Differentiation of induced pluripotent stem cells (iPSCs) into endoderm > zurl.co/kqo1B
The growth factors needed for this protocol can be purchased as a kit saving 36%; zurl.co/VT5l9
#iPSC #endoderm #stemcells
Online now! An evolutionary conserved role for EZH2-polycomb repressive complex 2 (PRC2) in safeguarding #pluripotency from primitive #endoderm cell fate. #hypoblast #transdifferentiation https://ow.ly/Q2eZ50XkBks
ISSCR | Cell Press | SickKids Foundation | The Gairdner Foundation
The LINC01612-DVL2-WNT regulatory axis promotes human endoderm differentiation – a key modulator of human definitive endoderm differentiation. #longnoncodingRNA #DVL2 #WNT #endoderm https://ow.ly/zvgJ50XhLB8
ISSCR | Cell Press | The Gairdner Foundation | SickKids Foundation
Epiblast Ca2+ waves propagate from the apical to basal domain. The images show selected time points from movies of E5.5 GCaMP6f embryos imaged using a ZEISS lattice light-sheet 7 microscope. Top row: An example of an epiblast wave involving a large proportion of the tissue captured by imaging a volume of the embryo at 20-s intervals. A total of 9/16 embryos showed at least one epiblast Ca2+ wave. Bottom row: Faster imaging at 5-s interval revealed that the Ca2+ wave in the epiblast can be preceded by a transient in a neighboring VE cell—this was observed in 5/19 events from 9 embryos (white arrowheads).
During #development, distal visceral #endoderm (DVE) cells migrate to shape A-P axis specification. @m-stower.bsky.social @srinivas-lab.bsky.social &co reveal the role of Ca2+ dynamics in this, showing that inhibition of #calcium transients causes DVE arrest @plosbiology.org 🧪 plos.io/4nHWxF4
Epiblast Ca2+ waves propagate from the apical to basal domain. The images show selected time points from movies of E5.5 GCaMP6f embryos imaged using a ZEISS lattice light-sheet 7 microscope. Top row: An example of an epiblast wave involving a large proportion of the tissue captured by imaging a volume of the embryo at 20-s intervals. A total of 9/16 embryos showed at least one epiblast Ca2+ wave. Bottom row: Faster imaging at 5-s interval revealed that the Ca2+ wave in the epiblast can be preceded by a transient in a neighboring VE cell—this was observed in 5/19 events from 9 embryos (white arrowheads).
During #development, distal visceral #endoderm (DVE) cells migrate to shape A-P axis specification. @m-stower.bsky.social @srinivas-lab.bsky.social &co reveal the role of Ca2+ dynamics in this, showing that inhibition of #calcium transients causes DVE arrest @plosbiology.org 🧪 plos.io/4nHWxF4
Epiblast Ca2+ waves propagate from the apical to basal domain. The images show selected time points from movies of E5.5 GCaMP6f embryos imaged using a ZEISS lattice light-sheet 7 microscope. Top row: An example of an epiblast wave involving a large proportion of the tissue captured by imaging a volume of the embryo at 20-s intervals. A total of 9/16 embryos showed at least one epiblast Ca2+ wave. Bottom row: Faster imaging at 5-s interval revealed that the Ca2+ wave in the epiblast can be preceded by a transient in a neighboring VE cell—this was observed in 5/19 events from 9 embryos (white arrowheads).
During #development, distal visceral #endoderm (DVE) cells migrate to shape A-P axis specification. @m-stower.bsky.social @srinivas-lab.bsky.social &co reveal the role of Ca2+ dynamics in this, showing that inhibition of #calcium transients causes DVE arrest @plosbiology.org 🧪 plos.io/4nHWxF4
💡Application Note: Differentiation of induced pluripotent stem cells (iPSCs) into endoderm > zurl.co/H0Euu
The growth factors needed for this protocol can be purchased as a kit saving 38% > zurl.co/frw2g
#iPSC #endoderm #stemcells
💡Application Note: Differentiation of induced pluripotent stem cells (iPSCs) into endoderm
Read the full application note > zurl.co/H0Euu
Save 38% by buying the differentiation kit > zurl.co/frw2g
#iPSC #endoderm #stemcells
Matrigel inhibits elongation and drives endoderm differentiation in aggregates of mouse embryonic stem cells
👉 buff.ly/YUFeNSZ
🥼 @atoosa_amel @mubeengoolam
#Matrigel #Endoderm #EvoDevo #mESCs #stembryo
☕Moghe et al. show that embryonic primitive #endoderm cells migrate towards the inner cell mass-cavity interface, depositing an #ExtracellularMatrix gradient that may guide migration. Primitive endoderm to epiblast ratios may enable robust patterning across #embryos and sizes.
go.nature.com/4iLjlAP
#NCB2024
In June, Meissner & co reported a lineage-tracing strategy to track #extraembryonic gut #endoderm cells over development; showed these cells are eventually eliminated in a p53-dependent manner and neighboring embryonic cells clear their remnants.
www.nature.com/articles/s41...
