High Throughput Hyperspectral and Multiplexed Super-Resolution Fluorescence Imaging by SP-STORM | Stefan Prechtl
๐ฆ๐ฃ-๐ฆ๐ง๐ข๐ฅ๐ (๐ฆ๐ฝ๐ฒ๐ฐ๐๐ฟ๐ฎ๐น-๐ฃ๐ต๐ฎ๐๐ผ๐ฟ ๐ฆ๐ง๐ข๐ฅ๐ ) ๐๐ณ๐ฆ๐๐ณ๐ช๐ฏ๐ต ๐ธ๐ฐ๐ณ๐ต๐ฉ ๐ข ๐ญ๐ฐ๐ฐ๐ฌ โ๐๐ถ๐ด๐ต ๐ง๐ต๐ฟ๐ผ๐๐ด๐ต๐ฝ๐๐ ๐๐๐ฝ๐ฒ๐ฟ๐๐ฝ๐ฒ๐ฐ๐๐ฟ๐ฎ๐น ๐ฎ๐ป๐ฑ ๐ ๐๐น๐๐ถ๐ฝ๐น๐ฒ๐
๐ฒ๐ฑ ๐ฆ๐๐ฝ๐ฒ๐ฟ-๐ฅ๐ฒ๐๐ผ๐น๐๐๐ถ๐ผ๐ป ๐๐น๐๐ผ๐ฟ๐ฒ๐๐ฐ๐ฒ๐ป๐ฐ๐ฒ ๐๐บ๐ฎ๐ด๐ถ๐ป๐ด ๐ฏ๐ ๐ฆ๐ฃ-๐ฆ๐ง๐ข๐ฅ๐ โ (๐๐ฐ๐ต๐ต ๐ฆ๐ต ๐ข๐ญ., ๐ฃ๐ช๐ฐ๐๐น๐ช๐ท, ๐๐ฆ๐ฑ๐ต 2025 โ https://lnkd.in/d5smSTUN ) A clever implementation of spectral-phasor analysis directly in hardware โ achieving simultaneous five-color super-resolution imaging within about a minute. By merging sine/cosine filter optics with dSTORM principles, the method reduces spectral crosstalk (<5 %) and removes the need for sequential channel switching. The hardware-level multiplexing is a decisive step toward practical multi-target SMLM, though the current proof remains 2D and relies on custom filter sets. ๐ ๐ต๐ข๐ฏ๐จ๐ช๐ฃ๐ญ๐ฆ ๐ข๐ฅ๐ท๐ข๐ฏ๐ค๐ฆ ๐ช๐ฏ ๐ฎ๐ถ๐ญ๐ต๐ช๐ฑ๐ญ๐ฆ๐น๐ฆ๐ฅ ๐ฑ๐ฉ๐ฆ๐ฏ๐ฐ๐ต๐บ๐ฑ๐ช๐ฏ๐จ โ ๐ฐ๐ฏ๐ฆ ๐ต๐ฉ๐ข๐ต ๐ค๐ฐ๐ถ๐ญ๐ฅ ๐ณ๐ฆ๐ด๐ฉ๐ข๐ฑ๐ฆ ๐ฉ๐ฐ๐ธ ๐ด๐ฑ๐ฆ๐ค๐ต๐ณ๐ข๐ญ ๐ช๐ฏ๐ง๐ฐ๐ณ๐ฎ๐ข๐ต๐ช๐ฐ๐ฏ ๐ช๐ด ๐ช๐ฏ๐ต๐ฆ๐จ๐ณ๐ข๐ต๐ฆ๐ฅ ๐ช๐ฏ๐ต๐ฐ ๐๐๐ ๐ธ๐ฐ๐ณ๐ฌ๐ง๐ญ๐ฐ๐ธ๐ด ๐ฐ๐ฏ๐ค๐ฆ ๐ด๐ค๐ข๐ญ๐ฆ๐ฅ ๐ต๐ฐ ๐ฅ๐ ๐ข๐ฏ๐ฅ ๐ญ๐ช๐ท๐ฆ ๐ช๐ฎ๐ข๐จ๐ช๐ฏ๐จ. #SPSTORM #SpectralImaging #SMLM #Multiplex #HighContent #FluorescenceMicroscopy #bioRxiv
Five-color SR in one shot โ strong step toward scalable multiplexed phenotyping.
Next challenge: 3D + live-cell stability.
#SPSTORM #MultiplexImaging #HCI
