Dorymyrmex bicolor engage in more social interactions than D. insanus. (A) Schematic of behavior method with pairs of ants filmed in a glass petri dish for 3 min and interactions coded in BORIS (Friard and Gamba, 2016). (B) Stills from recordings showing D. bicolor engaging in biting (left) and grooming (right) behaviors. In comparisons of nestmate interactions or non-nestmate interactions between species, D. bicolor interact more often than D. insanus (C). In comparisons of nestmate and non-nestmate behavior within species, D. bicolor non-nestmates display significantly more aggressive interactions (D) and significantly fewer affiliative interactions (E) than nestmates, an effect not detected in D. insanus. D. bicolor nestmates participate in antennation for longer duration bouts (F) and a greater amount of total time than non-nestmates (G); D. insanus nestmates and non-nestmates show comparable antennation bout duration and total time. (H) Dorymyrmex insanus display few trophallaxis events with no differences among nestmate or non-nestmate pairs, whereas D. bicolor nestmates engage in trophallaxis more frequently than non-nestmates. Statistical comparisons in (A–E,H) were made using a generalized linear model with a Poisson distribution followed by ANOVA and post hoc comparisons using Tukey’s Honestly Significant Difference. Statistical comparisons in F and G were made using pairwise Mann-Whitney U-test between non-nestmate and nestmate pairs of a species. *p < 0.05; ***p < 0.001; Color code for data point in (C–H): D. insanus cyan; D. bicolor blue.
🐜✨Did you know #colony size may shape how #ants sense and recognize each other? Godfrey et al show ant senses evolve in colonies. Check out their dataset and article at doi.org/10.25422/azu... and doi.org/10.3389/fevo.... Image: Godfrey et al. (2021). CC BY 4.0. #OpenData #OpenScience #SocialInsects