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Posts tagged #SpatialBio

It's been a minute but I found my way back to a conference; I'll be at AGBT this week representing @stellaromics.bsky.social to lend my experience communicating all things #spatialbio #bioinformatics #techbio #ai ! Looking forward to meeting new folks, please reach out if interested! 🧬🔬

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#RNAbiology #SpatialGeneExpression #RNAlocalization #Cellcycle #SpatialBio

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Integrated spatial proteomics of human PDAC uncovers an expanded tumour–immune– stroma spectrum with genomic associations Distinctively, pancreatic ductal adenocarcinoma (PDAC) consists of sparse tumour lesions intertwined with extensive desmoplastic stroma. The complexity of tumour–microenvironment interactions within t...

New from @hartjackson.bsky.social: 104‑plex #IMC on 3 serial PDAC sections, 200+ patients, whole-slide & cellular modes. Spatial proteomics guides LCM + genomics to define tumor–immune–stromal spectra. A tour de force.

biorxiv.org/content/10.1...

@standardbiology.bsky.social #SpatialBio #IMC

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A phase 2 trial of CXCR4 antagonism and PD1 inhibition in metastatic pancreatic adenocarcinoma reveals recruitment of T cells but also immunosuppressive macrophages Pancreatic ductal adenocarcinoma (PDAC) is characterized by dense stroma and myeloid-rich microenvironment that confer resistance to immunotherapies. Previous studies demonstrated that disrupting t...

@johnshopkinssph.bsky.social: Another excellent paper from @wonjinho.bsky.social uses #IMC to profile liver immune microenvironments during inflammation, revealing complex immune cell compositions.

www.tandfonline.com/doi/full/10....

@standardbiology.bsky.social #SpatialBio #Spatialproteomics

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Simultaneous expression of epithelial and immune cell markers in circulating tumor cells identified in patients with stage 4 breast cancer - Communications Medicine Higa et al. analyzed circulating cells expressing cancer and immune cell markers in breast cancer patients. Based on genomic and protein expression profiling, they show that the cells were unlike prev...

New from @usc.edu : Peter Kuhn & James Hicks use CyTOF + IMC to profile rare immune-like CTCs (CD45⁺/CK⁺) in metastatic breast cancer. A novel hybrid cell type with clinical potential.

www.nature.com/articles/s43...

#SpatialBio @standardbiology.bsky.social #ImagingMassCytometry

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Neutrophils drive vascular occlusion, tumour necrosis and metastasis - Nature Neutrophils actively induce tumour necrosis, driving vascular occlusion, pleomorphic necrosis and metastasis.

Two standout papers from @johnshopkinssph.bsky.social teams using IMC via the imaging program led by @wonjinho.bsky.social:

🧬 Neutrophils & tumor necrosis (Nature): www.nature.com/articles/s41...
🧬 UNG inhibition in CRC: pubmed.ncbi.nlm.nih.gov/40663394/

#SpatialBio @standardbiology.bsky.social

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Immune landscape of sarcoma patients treated with an autologous dendritic cell vaccine. e23530Background: The role of the sarcoma immune tumor microenvironment (TME) is a critical area of ongoing research, particularly to understand responses to immunotherapy. We previously conducted a p...

Nice to see #IMC and #Phenoplex coming together to investigate immune landscapes. Two great tastes that taste great together.

ascopubs.org/doi/abs/10.1...

@standardbiology.bsky.social @visiopharm.bsky.social #SpatialProteomics #SpatialBio

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Bodenmiller et al. showing that the consistency and quality of @standardbiology.bsky.social CyTOF and IMC technologies can provide immune profiling insights to clinicians —highlighting their real-world impact on patient management.

onlinelibrary.wiley.com/doi/10.1002/...

#SpatialBio

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And thank you to #ErikSodeblom of @dukecellbiology.bsky.social for hosting the 2nd installment of #SpatialBio talks!!

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Closing the 2nd #DukeSCIRIP Colloquium, @dukemedschool.bsky.social #SpatialBio talks cont'd... #AnoopPatel of @dukeneurosurgery.bsky.social on #GBM & #Xenium; #ErikaCrosby of @dukesurgery.bsky.social on Xenium & #PDAC; and #JohnHickey of @dukeubme.bsky.social on #SpatialOmics in #BrainNeckCancer 🧬🧠🥼

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And an additional thanks to @tatalab-duke.bsky.social for hosting the first #SpatialBio session!! 🧬

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An excellent paper by Joel Eliason, Jared K. Burks, Suhendan Ekmekcioglu et al on characterizing the spatial immune architecture in melanoma using imaging mass cytometry #IMC.

www.frontiersin.org/journals/imm...

@standardbiology.bsky.social @visiopharm.bsky.social #SpatialBio #ImagingMassCytometry

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Duke #SCIRIP Colloquium on May 12th 👏 Join us for a day of #singlecell, #bioinformatics, #spatialbio, #proteomics and more, with #Duke researchers & external guest speakers! Registration is free (and easy!) - just email DukeSCI@duke.edu 🧬🧠💻🧑‍🔬 Visit dmpi.duke.edu/duke-single-...

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An excellent paper by Daniel W Rosenberg et al using #IMC makes the cover of the issue of AACR Journals Cancer Prevention Research being handed out at #AACR25. Congratulations!

aacrjournals.org/cancerpreven...

#ImagingMassCytometry @standardbiology.bsky.social #SpatialBio

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On my way to Chicago for #AACR25! Hope to see a few of you there. If you can, stop by and check out the joint @standardbiology.bsky.social @visiopharm.bsky.social poster on AI analysis for highplex proteomic images.

www.abstractsonline.com/pp8/#!/20273...

#SpatialBio #IMC #Phenoplex

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Duke #SCIRIP Colloquium on May 12th 👏 Join us for a day of #singlecell, #bioinformatics, #spatialbio, #proteomics and more, with #Duke researchers & external guest speakers! Registration is free (and easy!) - just email DukeSCI@duke.edu 🧬🧠💻🧑‍🔬

Visit dmpi.duke.edu/duke-single-... for more information

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Glad to be part of such an open and collaborative community pushing #SpatialBio forward! Thanks for inviting and hosting us @kevinyamauchi.bsky.social @lucamarconato.bsky.social & Giovanni Palla 🙏

I focused on the intro notebook and improving usability for first time users ⚒️ check it out! ->

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2D, or not 2D? Investigating Vertical Signal Integrity of Tissue Slices Imaging-based spatially resolved transcriptomics can localise transcripts within cells in 3D. Cell egmentation precedes assignment of transcripts to cells and nnotation of cell function. However, cell...

2D, or not 2D? Investigating Vertical Signal Integrity of Tissue Slices.

www.biorxiv.org/content/10.1...

A much-needed paper for #SpatialBio (plus a fabulous title). Cells in tissue sections are highly overlapping (even in a 5 um section). Just being aware of it in regular 2D analysis is good.

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I love that the #SpatialBio feed is being used both by the microscopic biology spatial proteomics and spatial transcriptomics people and by the macroscopic biology spatial biodistribution people like crop dynamics or fish distributions across a landscape!

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Bridging the Gap: Insights from Mouse and Human Models in Pancreatic Cancer Research Guest Speaker: Marco Erreni, Ph D, Humanitas Research Hospital

Webinar: Bridging the Gap: Insights from Mouse and Human Models in Pancreatic Cancer Research. Guest Speaker: Marco Erreni, Ph D, Humanitas Milano

my.demio.com/ref/qABk0lBp...

#PDAC #SpatialBio #IMC #Multipleximaging #SpatialProteomics @standardbiology.bsky.social

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GitHub - camlab-bioml/starling: Segmentation error aware clustering for multiplexed imaging Segmentation error aware clustering for multiplexed imaging - camlab-bioml/starling

Software available here: github.com/camlab-bioml...

Great collaboration with @hartjackson.bsky.social with support from @sinaihealth.bsky.social @oicr.on.ca @uoft.bsky.social

#spatialbio #singlecell #multiplexedimaging #machinelearning

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Hey #genomics / #bioinformatics / #SpatialBio world! Any examples of successfully integrating custom count norm methods into a Seurat pipeline for #singlecell data? I’m trying to introduce cell area-based normalization for spatial data but I keep getting strange errors from RunUMAP/uwot 🧬🖥️

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I had not heard of DVP until two weeks ago, and now I see it everywhere :-)

Joe Yeong (NUS, don't think he's on Bluesky) is very excited about it. @aruthak.bsky.social #SpatialBio

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Wow! What a big day for #multipleximaging and #spatialbio! I am proud to have played a role in the genesis of this field in I-O. And more to come! @standardbiology.bsky.social

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#Multiomics2024 was amazing and I was very proud to be invited to speak for @standardbiology.bsky.social. Here’s the cartoon version.

Huge thanks to @aruthak.bsky.social for bringing it all together. #spatialbio

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