Models capturing different kinetics and relationships between the cell populations of interest. Model A: Independent homogeneous model. Each population (TN, CD95int, CD95hi) is independent and homogeneous. Model B: Independent heterogeneous model. Each population (TN, CD95int, CD95hi) is independent and allowed (but not constrained) to be heterogeneous. Model C: Forked differentiation model. Upon meeting cognate antigen, TN cells undergo a clonal burst (of size 2k); a fraction (f) differentiate into the CD95int pool with the remainder differentiating into the CD95hi pool. Model D: Linear differentiation model (CD95int first). Upon meeting cognate antigen, TN cells undergo a clonal burst (of size 2k) and differentiate into the CD95int pool which subsequently differentiate into the CD95hi pool. Model E: Linear differentiation model (CD95hi first). Upon meeting cognate antigen, TN cells undergo a clonal burst (of size 2k) and differentiate into the CD95hi pool which subsequently differentiate into the CD95int pool. Note for compactness, in the cartoons, CD95int TSCM are designated Tint and CD95hi TSCM are designated Thi. In every model each population was assumed to be of constant size. The models were fitted to the data to identify which model was most consistent with the experimental observations and to quantify the population parameters.
How do T stem cell-like memory (TSCM) cells act to maintain long-term #ImmuneMemory? This study reveals that human #TSCM cells comprise two distinct subpopulations with complementary roles; 1. Supporting clonal longevity. 2. Driving expansion & self-renewal @plosbiology.org 🧪 plos.io/4kQi0tL