Karyotype evolution in laboratory cultures of M. lignano. (A) Brightfield image of an adult worm. Scale bar: 100 µm. (B) Karyotype polymorphisms with an increasing number of large chromosomes that can be found in M. lignano. Scale bars: 10 µm. (C) Dynamics of karyotype polymorphisms present in 12 experimental subcultures over a period of 26 months. All subcultures started with only the 2n=8 karyotype. The bars indicate the percentage of subcultures that also contain the 2n=9 and 2n=10 karyotypes as a function of time.
Stijn Mouton & co present a flow cytometric approach to identify karyotypic polymorphisms in Macrostomum lignano cultures, demonstrate de novo chromosome duplications under laboratory conditions, and propose a solution for controlling culture karyotypes. 🪱
doi.org/10.1242/bio....
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