#CardiovascRes

Hao Yin

@haoyin.bsky.social

1 month ago

Fibroblast TGFβ signaling in systolic & diastolic🫀functions

Protective▶️Lean 🐭
Pathogenic▶️Diabetic db/db🐭

Evidence from
Fibroblast Tgfbr2 or Smad3 KO🐭

Speckle-tracking echo
👉Peak systolic/diastolic longitudinal endocardial/epicardial strain

#CardiovascRes 2024
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

1 month ago

What are Markers of arterial adventitial fibroblast?
PDGFRA
DPEP1

3 fibroblast subsets
CD55+
LOX+
CXCL14+

CD55+ adventitial fibroblasts are enriched in thoracic aorta & carotid artery, but rare in other large arteries

#CardiovascRes 2023
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

3 months ago

PRMT5 di-methylates H3R8 & H4R3▶️
⏬Myocardin-dependent #SmoothMuscleCell contractile gene transcription
⏫SMC proliferation/migration & intimal hyperplasia in injured🐭carotid artery

PRMT5 (& PRMT4) ⬆️in PDGBB-treated SMC &👤coronary #Atherosclerosis

#CardiovascRes 2023
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

3 months ago

PRMT5 methylates & stabilizes KLF4 protein▶️
⏫#SmoothMuscleCell proliferation/migration & intimal hyperplasia in injured🐭carotid artery

Evidence from #SmoothMuscleCell PRMT5 KO🐭 & Lentiviral PRMT5

PRMT5⬆️ in👤carotid stenosis & PDGF-BB-treated SMC

#CardioVascRes 2023
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

4 months ago

#Atherosclerosis Epitranscriptomics

Mettl3-YTHDF3⏫m6A-MTRFA mRNA in SMCs➡️
⏫cholesterol-induced Mφ-like phenotype

SMC Mettl3 KO🐭+HFD AAV-PCSK9➡️
⏬Lesion burden, necrotic core, Mφ
👉Abolishes SMC contribution to lesion!
👉Reverted by AAV9-SM22-MRTFA

#CardiovascRes 2025
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

4 months ago

#SmoothMuscleCell m6A Epitranscriptomics

METTL3 as a direct target gene of YY1

METTL3-IGF2BP1 stabilizes MYH11 MYLK2 TGFβ2 mRNAs

SMC Yy1 or Mettl3 KO🐭
⏬vasoconstriction
↔️vasorelaxation

AAV-SM22-METTL2 iv ⏫arterial contractility of SMC Yy1 KO🐭

#CardiovascRes 2025
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

9 months ago

Heart #Lymphangiogenesis

"Endogenous lymphangiogenesis⬇️TAC-induced 🫀 inflammation & perivascular fibrosis, delaying #HeartFailure development in C57BL/6 but not in Balb/c🐭"

anti-VEGFR3 Ab B6🐭
⏫perivasc fibrosis
⏫Mφ NK B

@hfpef-diva.bsky.social #CardiovascRes 2023
academic.oup.com/cardiovascre...

BioMassSpec

@realbiomassspec.bsky.social

10 months ago

Integrated proteomics identifies troponin I isoform switch as a regulator of a sarcomere-metabolism axis during cardiac regeneration AbstractAims. Adult mammalian cardiomyocytes have limited regenerative potential, and after myocardial infarction (MI), injured cardiac tissue is replaced

Integrated proteomics identifies troponin I isoform switch as a regulator of a sarcomere-metabolism axis during cardiac regeneration #CardiovascRes #MassSpec academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

1 year ago

DJ-1/PARK7 (causal gene of #ParkinsonDisease)⏬Diabetic #VascularCalcification

Acetylation (by NAT10)-Ubiquitination (by TRIM32) cascade degrades DJ-1▶️
⏫STAT1 & RAGE
⏫#SmoothMuscleCell Osteogenesis
⏫SMC MFGE8+ #MatrixVesicle▶️
⏫MV-collagen binding

#CardiovascRes 2024
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

1 year ago

#AlternativeSplicing #SmoothMuscleCell

RBPMS, coordinating with RBFOX2, mediates splicing events of SMC contractile, focal adhesion & actin cytoskeleton genes

234 skipped exon events shared by high-level RBPMS overexpression in hESC adult aorta

#CardiovascRes 2024
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

1 year ago

Pericytes in the brain & heart: functional roles & response to ischaemia & reperfusion

"pericyte-mediated capillary constriction plays a key role in restricting blood flow after ischaemia"

Also in #SkeletalMuscle, Kidney or Diabetic Islets?😆

#CardiovascRes 2024
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

1 year ago

#SmoothMuscleCell #Atherosclerosis

SMC CCN2/CTGF KO creates a striking #Glycosaminoglycan-depositing & LDL-entrapping, pro-atherogenic phenotype😎◀️What is the cellular mechanism?🧐

What a crazy Fatty mouse Aorta!🤯

#CardiovascRes 2024
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

1 year ago

Inactivation of🐭Ogg1, an essential #BaseExcisionRepair enzyme that repairs 8-oxoguanine ▶️

#ArterialStiffening without altered collagen fibril abundance

A very sophisticated #Matrisome related to #Aging-related #OxidativeDNADamage

#CardiovascRes 2024
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

1 year ago

Transcriptomes of #LiverSinusoidalEndothelialCell & #GlomerularEndothelialCell of BMP9 KO🐭

#ArterioVenousMalformation/AV Shunting in liver intestine uterine
Arterial dilation/tortuosity in lung brain

#CardiovascRes 2024
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

1 year ago

#AngiotensinConvertingEnzyme as a major regulator of Macrophage Lipid Metabolism
+a potential role of PPARα

ACE 10/10 mice are resistant to #Atherosclerosis, with ⏫M2-like CD206+IL10+ACE+ macrophages

So ACEi can exacerbate #Atherosclerosis???😧
#CardiovascRes 2023
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

1 year ago

#Hepatocyte #Mitchondria #Atherosclerosis

Mito-dependent/-independent roles of DRP1 in hepatic PCSK9 expression

Mdivi-1, a DRP1 inhibitor->
⏬SREBP-1c->⏬PCSK9 transcription
Modulating hepatocyte secretome->⏬PCSK9 secretion, ⏫PCSK9 degradation

#CardiovascRes 2021
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

1 year ago

#SmoothMuscleCell MMP3 mediates #ArterialCalcification in mice+Vitamin D3 (How?🧐)

MMP3 is the only MMP upregulated in calcifying rat aortic SMCs (Pi/Ca2+)

Raul Guzman lab #CardiovascRes 2024
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

2 years ago

αSMA+ #SmoothMuscleCell-TRAP;Ldlr−/−;ApoB100/100🐭▶️SMC Translatome in #Atherosclerosis

Novel atherosclerosis proteins
Spink5
Atp1a4
Gm13293
Hs6st2
Itih4
Smim3
Pcdh11x
No EndMT trait detected

#CardiovascRes 2024
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

2 years ago

Transcriptional driver ZBTB11 of #Cardiomyocyte degeneration (#Autophagy #Apoptosis) in PKP2 #ArrhythmogenicCardiomyopathy

Tomo-seq
RNAseq of 214 100-μm cryosections of👤LV wall
Epicardium-to-Endocardium🤠

#SpatialTranscriptomics

#CardiovascRes 2022
academic.oup.com/cardiovascre...

Hao Yin

@haoyin.bsky.social

2 years ago

Genetic (p16-3MR) vs Pharmacological (ABT263) #Senolysis in ApoE-/-🐭#Atherosclerosis

p16-3MR: No benefits
ABT263:⏬lesion👉senolyis+leukocyte depletion

Proliferating Mφ are also p16+;SAβGal+
🧐#Senescence in situ?

Martin Bennett lab #CardiovascRes 2022
academic.oup.com/cardiovascre...