Intragastric infection induced by wild-type (WT) SS1 observed through urease test, ureB PCR, colonization in gastric tissue. (A) RUT of infected gastric tissue; day 0, day 7 and day 14 with respective PBS control. (B) Confirmatory ureB PCR for the presence of H. pylori recovered from the gastric tissue of infected mice. M-100 bp marker, L1-positive control, L2-day 0 post infection, L3-day7 post infection, L4-14-day post infection, L5-PBS control. (C) Blood agar plates showing (i) plate containing no H. pylori colonies recovered from gastric tissue of 7 days post PBS inoculated mice (ii) plate containing H. pylori colonies recovered from gastric tissue of 7 days post SS1 infected mice. (D) Colonies recovered from mice of 0, 7- and 14-days post infection. Data represented here are the mean values +/-standard deviation (SD) of three independent experiments. The differences in day-wise response of each colonization assay were highly significant with respect to PBS control tissue. Statistical significance was found between 0-day, 7-day and 14-day infected mice tissue (***P<0.001; ns, non-significant).
Sanjib Das and team introduce an efficient mouse model for prophylactic and therapeutic studies using surgical intervention by directly injecting H. pylori inoculum into the stomach. This resulted in severe infection in 7 days. 🐭 🧪
doi.org/10.1242/bio....
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