#buddingyeast

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

3 months ago

Hey Bud!
#BuddingYeast

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

9 months ago

Metabolic tuning enables immediate adaptation to energy stress in yeast In Saccharomyces cerevisiae , glucose depletion induces metabolic reprogramming through widespread transcriptional and translational reorganization. We report that initial, very rapid translational si...

Cool!
#Metabolism #Reprogramming #Energy #Stress #NTP #Helicase #Translation #BuddingYeast

www.biorxiv.org/content/10.1...

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

10 months ago

A kinetic ruler controls mRNA poly(A) tail length Poly(A) tails of newly synthesized mRNAs have uniform lengths, arising through cooperation between the cleavage and polyadenylation complex (CPAC) and poly(A) binding proteins (PABPs). In the budding ...

A tail of two Nab2’s….
Very interesting! Congrats!
#Nab2 #PolyATail #Length #BuddingYeast #Scerevisiae
#RNA

www.biorxiv.org/content/10.1...

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

10 months ago

Transcription termination promotes splicing efficiency and fidelity in a compact genome Splicing of terminal introns is coupled to 3′-end processing by cleavage and polyadenylation (CPA) of mRNAs in mammalian genes. Whether this functional coupling is universally conserved across eukaryo...

Just heard a great seminar from Guillaume Chanfreau discussing this work! @guillaumechanfreau.bsky.social
#TranscriptionTermination #Splicing #Efficiency #RNA #3'endProcessing #Scerevisiae #BuddingYeast

www.biorxiv.org/content/10.1...

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

11 months ago

Parasitic plasmids are anchored to inactive regions of eukaryotic chromosomes through a nucleosome signal | The EMBO Journal imageimageThe naturally occurring S. cerevisiae 2µ plasmid is one of few documented cases of eukaryotic plasmids, undergoing equal distribution between mother and daughter cells during mitosis. Using ...

#2µ #Plasmid #BuddingYeast #Scerevisiae #Chromosome #Nucleosome

www.embopress.org/doi/full/10....

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

New Insights into nuclear import and nucleolar localization of yeast RNA exosome subunits The RNA exosome is a multiprotein complex essential for RNA maturation and degradation. In budding yeast, a nine-subunit protein complex (Exo9) associated with Rrp44 forms Exo10 in the cytoplasm and, ...

Nice! #RNAExosome #Localization #BuddingYeast

New Insights into #nuclear #import and #nucleolar #localization of yeast #RNA #exosome #subunits

Valdir Gomes Neto...Carla C. Oliveira
www.biorxiv.org/content/10.1...

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

RNA exosome-driven RNA processing instructs the duration of the unfolded protein response Abstract. Upon stresses, cellular compartments initiate adaptive programs meant to restore homeostasis. Dedicated to the resolution of transient perturbati

#RNAExosome #Rrp6 #RNAProcessing #Splicing #UPR #UnfoldedProteinResponse #BuddingYeast

academic.oup.com/nar/article/...

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

An elevated level of the mRNA exporter Mex67-Mtr2 in nuclear mRNPs impairs nuclear mRNA export In eukaryotes, a crucial step in gene expression is the export of the mRNA out of nucleus. The conserved mRNA exporter Mex67-Mtr2 (NXF1-NXT1 in human) is a key factor in this process. In S. cerevisiae...

Cool!
#Mex67 #Nuclear #mRNAExport #mRNA #Nab2 #Yra1 #Hpr1 #mRNP #BuddingYeast #RNA

www.biorxiv.org/content/10.1...

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

Yeast as a Tool to Understand the Significance of Human Disease-Associated Gene Variants At present, the great challenge in human genetics is to provide significance to the growing amount of human disease-associated gene variants identified by next generation DNA sequencing technologies. ...

#Disease #BuddingYeast #Tool

www.mdpi.com/2073-4425/12...

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

Defenders of the Transcriptome: Guard Protein-Mediated mRNA Quality Control in Saccharomyces cerevisiae Cell survival depends on precise gene expression, which is controlled sequentially. The guard proteins surveil mRNAs from their synthesis in the nucleus to their translation in the cytoplasm. Although...

#mRNA #QualityControl #BuddingYeast

www.mdpi.com/1422-0067/25...

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

Oncohistone H3 E97K mutation facilitates CENP-A mislocalization and chromosomal instability in budding yeast Abstract. Mislocalization of overexpressed CENP-A (Cse4 in budding yeast) contributes to chromosomal instability (CIN) in yeasts, flies, and human cells. O

#Oncohistone #H3 #Histone #Chromatin #Cancer #BuddingYeast

academic.oup.com/nar/article/...

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

Free introns of tRNAs as complementarity-dependent regulators of gene expression Nostramo et al. demonstrate that free introns of tRNAs (fitRNAs) are a previously unknown class of small noncoding regulatory RNAs that bind to complementary sequences on mRNA ORFs, leading to decay. ...

Cool! Congrats!

#tRNA #Intron #fitRNA #BuddingYeast

Free introns of tRNAs as complementarity-dependent regulators of gene expression: Molecular Cell www.cell.com/molecular-ce...

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

New reporters for monitoring cellular NMD A monthly journal publishing high-quality, peer-reviewed research on all topics related to RNA and its metabolism in all organisms

Nice!

#NMD #Reporter #Yeast #BuddingYeast #Rli1 #ABCE1

rnajournal.cshlp.org/content/earl...

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

RNA | Mobile

#dsRNA #BuddingYeast

Pervasive formation of double-stranded RNAs by overlapping sense/antisense transcripts in budding yeast mitosis and meiosis

Szachnowski et al.

m.rnajournal.cshlp.org/content/earl...

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

Formyl-methionine-mediated eukaryotic ribosome quality control pathway for cold adaptation Lee et al. describe the fMet-mediated ribosome quality control pathway. Cold exposure enhances cytosolic retention of Fmt1, promoting fMet translation. Nip1 then recognizes Nt-fMet and recruits Arf1, Hcr1, and Rli1 to stall and split ribosomes, forming stress granules that boost yeast cell resilience to cold stress by preventing toxic fMet-protein synthesis.

Interesting!

#Ribosome #QC #fMet #Scerevisiae #BuddingYeast #Cold

www.cell.com/molecular-ce...

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

Interesting!
#rDNA #Chromatin #Sir2 #Scerevisiae #BuddingYeast

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

#Scerevisiae #BuddingYeast

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

Nice! Excellent tools for #yeast #genomeengineering!
#CRISPR #Cas9 #Retron #Plasmid #Scerevisiae #buddingyeast

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

#BuddingYeast #RNAPII

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

The de novo design and synthesis of yeast chromosome XIII facilitates investigations on aging - Nature Communications The de novo design and synthesis of the Sc 2.0 chromosome XIII enable in-depth investigations into biology. Here the authors construct the 884 Kb synXIII of Saccharomyces cerevisiae to investigate rep...

www.nature.com/articles/s41...
#BuddingYeast #Scerevisiae #SyntheticBiology #Aging

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Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

Glad to have joined Bluesky yesterday! Great to have followed so many old and new friends/colleagues. Looking forward to all the great RNA biology and fun science chats!
#RNA #RNASky
P.S. Are there any Starter Packs for model organisms, especially, #BuddingYeast #Scerevisiae?

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Posts tagged #buddingyeast