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Yinyin Zhuang, @xyshi.bsky.social et al. introduce land-ExM, a superresolution approach that simultaneously maps protein and #lipid ultrastructure to reveal 3D interactions of #membrane #organelles and condensates. rupress.org/jcb/article/...

#ExM #Microscopy #StructuralBiology

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I agree, #ExM really changed my microscopy work…

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Yinyin Zhuang, @xyshi.bsky.social et al. introduce land-ExM, a superresolution approach that simultaneously maps protein and #lipid ultrastructure to reveal 3D interactions of #membrane #organelles and condensates. rupress.org/jcb/article/...

#ExM #Microscopy #StructuralBiology

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Really happy with our first expanded Paulinella picture! 😁
#ExM: @fburki.bsky.social
Confocal microscopy: @annewalraven.bsky.social

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Another #notTHECover unfortunately.

But this gorgeous, Tron-like vibe, drawn by the amazing @munafomarzia.bsky.social for our recent #ExM work with @gautamdey.bsky.social & @centriolelab.bsky.social will still be printed out in the lab.

Read here: www.cell.com/cell/fulltex...

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✨ Blinking #nanobodies that work for single-molecule localization 🔬
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

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Happy to #ExM them !
We had gotten some from Enviromental sampkes, but eother the cells were fully hidden in their shells and we coulsnt ExM at the time, or the shell fully detached.
One way to bypass this, is Cryofixation and a culture packed with cells ?
Do you have a culture ?

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Should we #ExM them or what ?

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It is amazing that @dudinlab.bsky.social beautiful #ExM composition has been selected to highlight our #6mA work for the cover of @natgenet.nature.com. #Protists (and ichthyosporeans in particular) in the cover of NG must be quite unique!

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NHSester in #ExM !!!

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As your sample expands, our dyes stay bright!

#Centrioles of #Chlamydomonas, 4x gel-expanded and imaged with confocal and STED, using #FLEXPOSURE to reduce bleaching. Staining: PolyE (abberior STAR RED).

Sample courtesy: P. Guichard & D. Gambarotto, Uni of Geneva

#ExM #fluorescencefriday

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Had a fun two weeks in Heidelberg visiting in the @floravincent.bsky.social and @gautamdey.bsky.social labs! Expanding diatoms and their evil little parasites was peak Halloween vibes 🎃 @embl.org #ExM #diatoms

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🔬 Community Workshop #2: "Stretching the Limits: Exploring Biology with Expansion Microscopy"

Learn how to unlock nanoscale #imaging with everyday microscopes using Expansion #Microscopy#ExM.

🧑‍🔬Led by experts from @itqbnova.bsky.social: Carolina Feliciano and @mariodelr.bsky.social.

#SPAOM2025

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#ExM #3DSTED for #fluorescencefriday:
#herpesvirus particles 4-5x expanded. Viral glycoprotein (🟨STAR RED) and tegument protein (🟦STAR 580) stained.
Don’t just expand your sample, expand your options with abberior dyes and STED!
Sample courtesy: Jannik Jensen & @bosse-lab.bsky.social
#virology

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Nieuw Binnen Bij Riddim Record Store!

#EefjedeVisser
#TameImpala
#Madonna
#Normaal
#Rare40thAnniversaryCollection
#TheLastDinnerParty
#WithinTemptation
#Sabaton
#PommelienThijs
#SuicideboyS
#TheWarOnDrugs
#SammyVirji
#Oasis
#Toto
#Blawan
#RoelFuncken
#Exm
#PolaandBryson

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Mesoscale volumetric fluorescence imaging at nanoscale resolution by photochemical sectioning Optical nanoscopy of intact biological specimens has been transformed by recent advancements in hydrogel-based tissue clearing and expansion, enabling the imaging of cellular and subcellular structure...

www.science.org/doi/10.1126/...

Cover: 3D WT bulb, wedge-out view; VIPS alternates light-sheet imaging & photochemical sectioning. #ExM #LLSM #imaging #neuroscience

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Our collaboration with @mbonhivers.bsky.social is now out in @plosbiology.org! 🎉Using U-ExM, we mapped the formation of the flagellar pocket collar in T. brucei. Huge thanks to everyone involved — one step closer to #PhDone!

#ExM #oneringtorulethemall #Bilbo1 #trypanosoma #protistsonsky

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Helge Ewers pointing towards a huge screen that shows different stages of cytokinesis;  Illustrated by individual and averaged U-ExM images of Septin2-GFP (green) and tubulin (magenta)

Helge Ewers pointing towards a huge screen that shows different stages of cytokinesis; Illustrated by individual and averaged U-ExM images of Septin2-GFP (green) and tubulin (magenta)

Our very own @ewerslab.bsky.social presents our story on Multiscale imaging of #Cytokinesis, harnessing #ExM 🔬🫧 of cytokinetic bridges 👀 🍬at this year's #BiomembraneDays25 👏🏽.
Read more? 👉🏽 doi.org/10.1101/2025...

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Sketches of six stages of cytokinetic development of the intercellular bridge are illustrated with exmaple U-ExM fluorescence microscopy images. Septin2-GFP shown in green. Tubulin shown in magenta. A shortened workflow illustrates the image alignment and averaging process to generate average 2D reconstructions of cytokinetic stages.

Sketches of six stages of cytokinetic development of the intercellular bridge are illustrated with exmaple U-ExM fluorescence microscopy images. Septin2-GFP shown in green. Tubulin shown in magenta. A shortened workflow illustrates the image alignment and averaging process to generate average 2D reconstructions of cytokinetic stages.

It is out! 🦚 I recorded hundreds of #ExM 🔬images of cytokinetic bridges and averaged them into 6 stages. How? With help from our fantastic collaborators @zacsimile.bsky.social and @jonasries.bsky.social in Vienna 😇. Check out the full atlas here: doi.org/10.5281/zenodo.17232370

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🔬✨ #GEF25 Expansion Microscopy Forum in Göttingen just wrapped up! We were happy to support as sponsors and share our sdAb innovations for super-resolution #microscopy. Thanks to the organizers & all participants for an inspiring event! 👏

#ExM #SmartSecondaries #Multiplexing

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Fantastic work by Nadia and co in the @ewerslab.bsky.social on recapitulating the dynamics of cytokinesis with #ExM!

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Taking a break from my thesis marathon and enjoying the lecture by @eboyden3.bsky.social at the #GEF25 😄. A wonderful ride through the beginnings, present and future of #ExM 🔬. What a great opportunity to join via live stream, big thanks to the organizers @alishaib.bsky.social and Silvio Rizzoli 😊

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Catch an #ExM talk with my @the.3i.social colleague @dvonwangenheim.bsky.social
at #GEF25 (streaming online) starting at 17:00 CEST. Excited to take part in this conference on an exciting application & in our European headquarters of Göttingen, Germany. Live stream: www.youtube.com/@neuroscienc...

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Now out on bioRxiv. 🥳My research on #cytokinesis, averaging thousands of #ExM images🔬, creating a dynamic atlas of cytokinesis 🦠⏳. Here's an animated sneak peek of what we found. Better resolution on bioRxiv😄 #PSFoftheGIF

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See us at Göttingen #ExM Forum #GEF25 and check out how expansion, abberior STAR dyes, and superresolution STED microscopy can team up to boost your imaging. We look forward to talking to you!

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Speakers wear red,
Attendees wear blue,
ExM’s biggest gathering
Is waiting for you.

#GEF25 — the #ExM event of the decade. Just 8days away!
Partners: @abberior.rocks & Wiley

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#ExM rocks!!!

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#ExM turned 10 this year - just like #NanoTag! 🎉
Join us & the super-resolution community in Göttingen for the ExM Forum! 🔬Hear from our founder Felipe Opazo @opazo.bsky.social and CSO Steffen Frey on how NanoTag’s sdAb solutions empower nanoscopy & #multiplex imaging.

See you in Göttingen!
#GEF25

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QRP with your wings art! 🦋

#art #ff14 #ffxiv #exm #catboy

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📄 🌟 Researchers introduce HAK-actin, an actin label compatible with post-labeling in #SuperResolution ultrastructure expansion #Microscopy #ExM. The authors demonstrate its use in cells expanded up to 16-fold (figure).

🔗 View the preprint article: bit.ly/47xC8NJ

#Microscope #Imaging #Fluorescence

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